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- Table of Contents
LYZ antibody (PB9663) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IF analysis of Lysozyme using anti-Lysozyme antibody (PB9663).
Lysozyme was detected in paraffin-embedded section of human ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Lysozyme was detected in paraffin-embedded section of human ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Lysozyme/LYZ Antibody Picoband®
LYZ antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
LYZ antibody was positively detected by WB in:
human THP-1 whole cell, human HL-60 whole cell, rat lung tissuerat PC-12 whole cellmouse lung tissuemouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LYZ antibody was positively detected by IHC in:
human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LYZ antibody was positively detected by IF in:
human ileum tissue, human colon organoid tissue, mouse ileum tissue, mouse ileum organoid tissue, mouse colon issue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunofluorescence
5μg/ml
Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Mouse
Recommend
Tested
Product Information
Product Name
Anti-Lysozyme/LYZ Antibody Picoband®
View all LYZ Antibodies
View all LYZ Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Lysozyme.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15-17 kDa
Calculated Molecular Weight
16.5 kDa
Background of LYZ
In humans, the lysozyme enzyme is encoded by the LYZ gene. This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta [1-4] glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
Cite This Product
Anti-Lysozyme/LYZ Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9663)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
89%
Moderate Homology
Mouse
75%
Moderate Homology
Rabbit
75%
Moderate Homology
Sheep
75%
Moderate Homology
Dog
75%
Moderate Homology
Cow
72%
Moderate Homology
Rat
67%
Low homology
Drosophila
33%
Low homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LYZ antibody
Immunohistochemistry (IHC) protocol for LYZ antibody
Immunofluorescence (IF) protocol for LYZ antibody
Publications
Application:
Species:
Integration of Multi-Omics and Network Pharmacology Analysis Reveals the Mechanism of Qingchang Huashi Jianpi Bushen Formula in Repairing the Epithelial Barrier of Ulcerative Colitis
Published: 2025-05-13
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 40386180
Dynamic changes and mechanism of intestinal endotoxemia in partially hepatectomized rats
Published: 2007-07-14
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IHC
Impact Factor: 0.00
Species: Rat
PMID: 17659709
Review
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Product Promise
We promise Anti-Lysozyme/LYZ Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9663
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026