Product Info Summary
SKU: | A02479 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-LysRS KARS Monoclonal Antibody
SKU/Catalog Number
A02479
Size
100μl
Form
Liquid
Description
Boster Bio Anti-LysRS KARS Monoclonal Antibody catalog # A02479. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LysRS KARS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02479)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8G12C1
Isotype
IgG
Immunogen
Purified recombinant fragment of LysRS (aa90-174) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02479 is reactive to KARS1 in Human
Applications
A02479 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
68.048kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using LysRS Monoclonal antibody against truncated Trx-LysRS recombinant protein (1), truncated MBP-LysRS (aa90-174) and full length LysRS (aa1-188) transfected CHO-K1 cell lysate (3).
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using LysRS Monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For KARS1 (Source: Uniprot.org, NCBI)
Gene Name
KARS1
Full Name
Lysine--tRNA ligase
Weight
68.048kDa
Superfamily
class-II aminoacyl-tRNA synthetase family
Alternative Names
Lysine--tRNA ligase KARS1 CMTRIB, DEAPLE, DFNB89, KARS, KARS2, KRS, LEPID lysyl-tRNA synthetase 1 lysine--tRNA ligase|lysRS|lysine tRNA ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KARS1, check out the KARS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KARS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LysRS KARS Monoclonal Antibody (A02479)
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No publications found for A02479
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-LysRS KARS Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thoracic mammary gland using anti-LysRS Monoclonal antibody A02479. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-31
Question
We are currently using anti-LysRS Monoclonal antibody A02479 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The anti-LysRS Monoclonal antibody (A02479) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-10
Question
I have attached the WB image, lot number and protocol we used for thoracic mammary gland using anti-LysRS Monoclonal antibody A02479. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-LysRS Monoclonal antibody (A02479)?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
We do provide the blocking peptide for product anti-LysRS Monoclonal antibody (A02479). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-14
Question
I was wanting to use your anti-LysRS Monoclonal antibody for IHC for human thoracic mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thoracic mammary gland identification?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
It shows on the product datasheet, A02479 anti-LysRS Monoclonal antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thoracic mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-01