Anti-Transcription factor MafF MAFF Antibody

Maff antibody

Boster Bio Anti-Transcription factor MafF MAFF Antibody (Catalog # A07784). Tested in IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A07784
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC

Product Name

Anti-Transcription factor MafF MAFF Antibody

View all Maff Antibodies

SKU/Catalog Number

A07784

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Transcription factor MafF MAFF Antibody (Catalog # A07784). Tested in IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Transcription factor MafF MAFF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07784)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MAFF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07784 is reactive to Maff in Human, Mouse

Applications

A07784 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

16.955kDa

Background of Maff

Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response.

Ota T., Nat. Genet. 36:40-45(2004).
Moran J.A., Biochem. J. 361:371-377(2002).
Dunham I., Nature 402:489-495(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For Maff (Source: Uniprot.org, NCBI)

Gene Name

Maff

Full Name

Transcription factor MafF

Weight

16.955kDa

Superfamily

bZIP family

Alternative Names

hMafF; MafF; transcription factor MafF; U-Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F; v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog F Maff|v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian)|transcription factor MafF|V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Maff, check out the Maff Infographic

Maff infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Maff: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Transcription factor MafF MAFF Antibody

Question

Does A07784 anti-MAFF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

As indicated on the product datasheet, A07784 anti-MAFF antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-02

Question

Here is the WB image, lot number and protocol we used for amniotic fluid using anti-MAFF antibody A07784. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-12

Question

Our lab want to know about to test anti-MAFF antibody A07784 on mouse amniotic fluid for research purposes, then I may be interested in using anti-MAFF antibody A07784 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Dhar

Verified customer

Asked: 2019-09-18

Answer

The products we sell, including anti-MAFF antibody A07784, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-18

Question

Does anti-MAFF antibody A07784 work for IHC with amniotic fluid?

O. Jones

Verified customer

Asked: 2019-06-17

Answer

According to the expression profile of amniotic fluid, MAFF is highly expressed in amniotic fluid. So, it is likely that anti-MAFF antibody A07784 will work for IHC with amniotic fluid.

Boster Scientific Support

Answered: 2019-06-17

Question

I see that the anti-MAFF antibody A07784 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-04-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-04-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-MAFF antibody A07784. Let me know if you need anything else.

R. Mitchell

Verified customer

Asked: 2017-05-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-18

Question

We are currently using anti-MAFF antibody A07784 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?

T. Carter

Verified customer

Asked: 2013-09-02

Answer

The anti-MAFF antibody (A07784) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-09-02

Order DetailsPrice
A07784-100uL

100uL

$360

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A07784
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