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Product Info Summary
| SKU: | A01524 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MAP1LC3B Antibody
SKU/Catalog Number
A01524
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MAP1LC3B Antibody (Catalog # A01524). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MAP1LC3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01524)
Host
Rabbit
Contents
MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. The immunogen is located within the first 50 amino acids of MAP1LC3B.
Cross-reactivity
MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C
Reactive Species
A01524 is reactive to MAP1LC3B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
14.7 kDa
Background of MAP1LC3B
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01524 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 μg/ml.
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Immunohistochemistry of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 5 μg/mL.
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Immunofluorescence of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 20 μg/mL.
Specific Publications For Anti-MAP1LC3B Antibody (A01524)
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