Product Info Summary
SKU: | PB9356 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MASPIN/SERPINB5 Antibody Picoband™
View all Serpin B5/Maspin Antibodies
SKU/Catalog Number
PB9356
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MASPIN/SERPINB5 Antibody Picoband™ catalog # PB9356. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MASPIN/SERPINB5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9356)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9356 is reactive to SERPINB5 in Human, Mouse, Rat
Applications
PB9356 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.1kDa
Background of Serpin B5/Maspin
SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-MASPIN Picoband antibody, PB9356, Western blotting
All lanes: Anti MASPIN (PB9356) at 0.5ug/ml
Lane 1: Mouse Intestine Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Click image to see more details
Anti-MASPIN Picoband antibody, PB9356, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Click image to see more details
Anti-MASPIN Picoband antibody, PB9356, IHC(P)
IHC(P): Rat Intestine Tissue
Click image to see more details
Anti-MASPIN Picoband antibody, PB9356, IHC(P)
IHC(P): Mouse Intestine Tissue
Click image to see more details
Figure 5. IHC analysis of MASPIN using anti-MASPIN antibody (PB9356).
MASPIN was detected in immunocytochemical section of MCF-7 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MASPIN Antibody (PB9356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. Western blot analysis of MASPIN using anti-MASPIN antibody (PB9356).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat small intestine tissue lysates,
Lane 2: mouse small intestine tissue lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human MDA-MB-231 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASPIN antigen affinity purified polyclonal antibody (Catalog # PB9356) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42KD. The expected band size for MASPIN is at 42KD.
Protein Target Info & Infographic
Gene/Protein Information For SERPINB5 (Source: Uniprot.org, NCBI)
Gene Name
SERPINB5
Full Name
Serpin B5
Weight
42.1kDa
Superfamily
serpin family
Alternative Names
Maspin; Peptidase inhibitor 5; PI5; PI-5; PI5protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin peptidase inhibitor, clade B (ovalbumin), member 5 SERPINB5 PI5, maspin serpin family B member 5 serpin B5|PI-5|peptidase inhibitor 5|protease inhibitor 5 (maspin)|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5|serpin peptidase inhibitor, clade B (ovalbumin), member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINB5, check out the SERPINB5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MASPIN/SERPINB5 Antibody Picoband™ (PB9356)
Hello CJ!
PB9356 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line
Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer
Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MASPIN/SERPINB5 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-MASPIN/SERPINB5 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-MASPIN/SERPINB5 Antibody Picoband™
Question
Will anti-MASPIN/SERPINB5 antibody PB9356 work on monkey WB with mammary gland?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Our lab technicians have not validated anti-MASPIN/SERPINB5 antibody PB9356 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-MASPIN/SERPINB5 antibody PB9356 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey mammary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
I have attached the WB image, lot number and protocol we used for squamous epithelium using anti-MASPIN/SERPINB5 antibody PB9356. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for squamous epithelium using anti-MASPIN/SERPINB5 antibody PB9356. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
We are currently using anti-MASPIN/SERPINB5 antibody PB9356 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
J. Dhar
Verified customer
Asked: 2019-09-03
Answer
The anti-MASPIN/SERPINB5 antibody (PB9356) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
I was wanting to use your anti-MASPIN/SERPINB5 antibody for IHC-P for human squamous epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human squamous epithelium identification?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
As indicated on the product datasheet, PB9356 anti-MASPIN/SERPINB5 antibody has been tested for IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human squamous epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-11
Question
Is this PB9356 anti-MASPIN/SERPINB5 antibody reactive to the isotypes of SERPINB5?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
The immunogen of PB9356 anti-MASPIN/SERPINB5 antibody is E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-04