Anti-MASPIN/SERPINB5 Antibody Picoband™

Serpin B5/Maspin antibody

Boster Bio Anti-MASPIN/SERPINB5 Antibody Picoband™ catalog # PB9356. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: PB9356
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

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Product Name

Anti-MASPIN/SERPINB5 Antibody Picoband™

View all Serpin B5/Maspin Antibodies

SKU/Catalog Number

PB9356

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MASPIN/SERPINB5 Antibody Picoband™ catalog # PB9356. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MASPIN/SERPINB5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9356)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9356 is reactive to SERPINB5 in Human, Mouse, Rat

Applications

PB9356 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

42.1kDa

Background of Serpin B5/Maspin

SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For SERPINB5 (Source: Uniprot.org, NCBI)

Gene Name

SERPINB5

Full Name

Serpin B5

Weight

42.1kDa

Superfamily

serpin family

Alternative Names

Maspin; Peptidase inhibitor 5; PI5; PI-5; PI5protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin peptidase inhibitor, clade B (ovalbumin), member 5 SERPINB5 PI5, maspin serpin family B member 5 serpin B5|PI-5|peptidase inhibitor 5|protease inhibitor 5 (maspin)|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5|serpin peptidase inhibitor, clade B (ovalbumin), member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SERPINB5, check out the SERPINB5 Infographic

SERPINB5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SERPINB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9356 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line

Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer

Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.

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6 Customer Q&As for Anti-MASPIN/SERPINB5 Antibody Picoband™

Question

Will anti-MASPIN/SERPINB5 antibody PB9356 work on monkey WB with mammary gland?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

Our lab technicians have not validated anti-MASPIN/SERPINB5 antibody PB9356 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-MASPIN/SERPINB5 antibody PB9356 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey mammary gland in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-25

Question

I have attached the WB image, lot number and protocol we used for squamous epithelium using anti-MASPIN/SERPINB5 antibody PB9356. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-15

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for squamous epithelium using anti-MASPIN/SERPINB5 antibody PB9356. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-08

Question

We are currently using anti-MASPIN/SERPINB5 antibody PB9356 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

J. Dhar

Verified customer

Asked: 2019-09-03

Answer

The anti-MASPIN/SERPINB5 antibody (PB9356) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-03

Question

I was wanting to use your anti-MASPIN/SERPINB5 antibody for IHC-P for human squamous epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human squamous epithelium identification?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

As indicated on the product datasheet, PB9356 anti-MASPIN/SERPINB5 antibody has been tested for IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human squamous epithelium in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-11

Question

Is this PB9356 anti-MASPIN/SERPINB5 antibody reactive to the isotypes of SERPINB5?

Verified Customer

Verified customer

Asked: 2018-06-04

Answer

The immunogen of PB9356 anti-MASPIN/SERPINB5 antibody is E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-04

Order DetailsPrice
PB9356

100μg

$370
PB9356-10ug

10μg sample (liquid)

$99
PB9356-Biotin

100 μg Biotin conjugated

$570
PB9356-Cy3

100 μg Cy3 conjugated

$570
PB9356-Dylight488

100 μg Dylight488 conjugated

$570
PB9356-Dylight550

100 μg Dylight550 conjugated

$570
PB9356-Dylight594

100 μg Dylight594 conjugated

$570
PB9356-FITC

100 μg FITC conjugated

$570
PB9356-HRP

100 μg HRP conjugated

$570
PB9356-APC

100 μg APC conjugated

$670
PB9356-PE

100 μg PE conjugated

$670
PB9356-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9356
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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