Product Info Summary
SKU: | A02336-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-MBD1 Antibody Picoband™
SKU/Catalog Number
A02336-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MBD1 Antibody Picoband™ catalog # A02336-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02336-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MBD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02336-1 is reactive to MBD1 in Human, Mouse, Rat
Applications
A02336-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
67 kDa
Calculated molecular weight
66.607kDa
Background of MBD1
MBD1 (Methyl-CpG-Binding Domain Protein 1), also known as PCM1 or CXXC3, is a protein that in humans is encoded by the MBD1 gene. Using PCR on a hybrid panel and FISH, Hendrich et al. (1999) mapped the MBD1 gene to chromosome 18q21, 2.1 cM distal to MBD2. Using yeast 2-hybrid analysis, reciprocal immunoprecipitation analysis, and protein pull-down assays, Fujita et al. (2003) showed that MBD1 interacted directly with MCAF. Deletion analysis revealed that the C-terminal transcriptional repressor domain (TRD) of MBD1 interacted with a conserved C-terminal domain of MCAF. Reporter gene assays showed that MCAF increased the repressive function of the isolated TRD of MBD1 against SP1. Chromatin immunoprecipitation analysis revealed that MBD1 linked MCAF to methylated promoters. Uchimura et al. (2006) found that MBD1 was multiply sumoylated in HeLa cells. Sumoylation did not alter the intracellular localization of MBD1 at nuclear foci in C-33A human cervical cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MBD1 using anti-MBD1 antibody (A02336-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HELA whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human U20S whole cell lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD1 antigen affinity purified polyclonal antibody (Catalog # A02336-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD1 at approximately 67KD. The expected band size for MBD1 is at 67KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of A549 cells using anti-MBD1 antibody (A02336-1).
Overlay histogram showing A549 cells stained with A02336-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MBD1 Antibody (A02336-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of U20S cells using anti-MBD1 antibody (A02336-1).
Overlay histogram showing U20S cells stained with A02336-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MBD1 Antibody (A02336-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MBD1 (Source: Uniprot.org, NCBI)
Gene Name
MBD1
Full Name
Methyl-CpG-binding domain protein 1
Weight
66.607kDa
Alternative Names
CXXC-type zinc finger protein 3; methyl-CpG binding domain protein 1 isoform PCM1; methyl-CpG binding domain protein 1; methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM1CXXC3RFT; Protein containing methyl-CpG-binding domain 1; the regulator of fibroblast growth factor 2 (FGF-2) transcription MBD1 CXXC3, PCM1, RFT methyl-CpG binding domain protein 1 methyl-CpG-binding domain protein 1|CXXC-type zinc finger protein 3|protein containing methyl-CpG-binding domain 1|the regulator of fibroblast growth factor 2 (FGF-2) transcription
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD1, check out the MBD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD1 Antibody Picoband™ (A02336-1)
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No publications found for A02336-1
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