Anti-MBD3 Antibody

Product Name

Anti-MBD3 Antibody

View all Mbd3 Antibodies

SKU/Catalog Number

A02571

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MBD3 Antibody (Catalog # A02571). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MBD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02571)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human MBD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02571 is reactive to Mbd3 in Human, Mouse

Applications

A02571 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

32.168kDa

Background of Mbd3

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (). Recruits histone deacetylases and DNA methyltransferases.

Hendrich B., Mol. Cell. Biol. 18:6538-6547(1998).
Grimwood J., Nature 428:529-535(2004).
Tatematsu K., Genes Cells 5:677-688(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from Jurkat cells, using MBD3 antibody A02571.

Gene/Protein Information For Mbd3 (Source: Uniprot.org, NCBI)

Alternative Names

MBD3; methyl-CpG binding domain protein 3; methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3 Mbd3|AI181826, AU019209|methyl-CpG binding domain protein 3|methyl-CpG-binding domain protein 3|methyl-CpG-binding protein MBD3

For more info on Mbd3, check out the Mbd3 Infographic

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In this infographic, you will see the following information for Mbd3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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