Anti-Mcl-1 Antibody

Product Name

Anti-Mcl-1 Antibody

View all Mcl-1 Antibodies

SKU/Catalog Number

A00712

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Mcl-1 Antibody (Catalog # A00712). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Mcl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Mcl-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00712)

Host

Rabbit

Contents

Mcl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Mcl-1. The immunogen is located within amino acids 220 - 270 of Mcl-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This Mcl-1 antibody detects isoforms Mcl-1L and Mcl-1ES.

Reactive Species

A00712 is reactive to MCL1 in Human, Mouse, Rat

Applications

A00712 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

37.337kDa

Background of Mcl-1

Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1ES by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Mcl-1 in Raji cell lysate with Mcl-1 antibody at 0.5 μg/mL.

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Immunocytochemistry staining of Raji cells using Mcl-1 antibody at 2 μg/mL.

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Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 10 μg/mL.

Gene/Protein Information For MCL1 (Source: Uniprot.org, NCBI)

Alternative Names

BCL2L3; bcl2-L-3; BCL2L3MGC104264; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MGC1839; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); TM MCL1 BCL2L3, EAT-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT, MCL1 MCL1 apoptosis regulator, BCL2 family member induced myeloid leukemia cell differentiation protein Mcl-1|BCL2 family apoptosis regulator|MCL1, BCL2 family apoptosis regulator|bcl-2-like protein 3|bcl-2-related protein EAT/mcl1|myeloid cell leukemia 1|myeloid cell leukemia ES|myeloid cell leukemia sequence 1 (BCL2-related)

For more info on MCL1, check out the MCL1 Infographic

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In this infographic, you will see the following information for MCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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