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Product Info Summary
| SKU: | A00712-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MCL1 Antibody (BH3 Domain Specific)
SKU/Catalog Number
A00712-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-MCL1 Antibody (BH3 Domain Specific) (Catalog # A00712-1). Tested in IHC-P, WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MCL1 Antibody (BH3 Domain Specific) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00712-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-226 amino acids from human MCL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00712-1 is reactive to MCL1 in Human, Mouse, Rat
Applications
A00712-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
37.337kDa
Background of Mcl-1
The Mcl-1 protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:10-1:50
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-MCL1 Antibody (BH3 Domain Specific) at 1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: Ramos whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: F9 whole cell lysate
Lane 5: A20 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-MCL1 Antibody (BH3 Domain Specific) at 1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: Ramos whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: F9 whole cell lysate
Lane 5: rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Overlay histogram showing A431 cells stained with A00712-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A00712-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For MCL1 (Source: Uniprot.org, NCBI)
Gene Name
MCL1
Full Name
Induced myeloid leukemia cell differentiation protein Mcl-1
Weight
37.337kDa
Superfamily
Bcl-2 family
Alternative Names
BCL2L3; bcl2-L-3; BCL2L3MGC104264; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MGC1839; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); TM MCL1 BCL2L3, EAT-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT, MCL1 MCL1 apoptosis regulator, BCL2 family member induced myeloid leukemia cell differentiation protein Mcl-1|BCL2 family apoptosis regulator|MCL1, BCL2 family apoptosis regulator|bcl-2-like protein 3|bcl-2-related protein EAT/mcl1|myeloid cell leukemia 1|myeloid cell leukemia ES|myeloid cell leukemia sequence 1 (BCL2-related)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCL1, check out the MCL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCL1 Antibody (BH3 Domain Specific) (A00712-1)
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No publications found for A00712-1
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