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Product Info Summary
| SKU: | M02580-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-MCM3 Antibody
SKU/Catalog Number
M02580-1
Size
50 µl
Description
Boster Bio Anti-MCM3 Antibody (Catalog # M02580-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MCM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02580-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1593CT377.41.73
Isotype
IgG1,k
Immunogen
This MCM3 antibody is generated from a mouse immunized with a recombinant protein of human MCM3.
Reactive Species
M02580-1 is reactive to MCM3 in Human, Mouse, Rat
Calculated molecular weight
91.0 kDa
Background of MCM3
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02580-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-MCM3 Antibody at 1:4000 dilution
Lane 1: CHO whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: C2C12 whole cell lysate
Lane 6: PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 91 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with M02580-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M02580-1 , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-MCM3 Antibody (M02580-1)
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