Product Info Summary
SKU: | A01688-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MCPIP1/ZC3H12A Antibody Picoband™
View all MCPIP1/ZC3H12A Antibodies
SKU/Catalog Number
A01688-2
Size
100 μg/vial
Form
Lyophilized
Description
Rabbit IgG polyclonal antibody for MCPIP1/ZC3H12A detection. Tested with WB, IHC, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCPIP1/ZC3H12A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01688-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MCPIP1/ZC3H12A recombinant protein (Position: Q36-T98).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01688-2 is reactive to ZC3H12A in Human, Mouse, Rat
Applications
A01688-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
73 kDa
Calculated molecular weight
65.699kDa
Background of MCPIP1/ZC3H12A
Zinc finger CCCH-type containing 12A is a protein in humans that is encoded by the ZC3H12A gene. ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 5-10μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MCPIP1/ZC3H12A using anti-MCPIP1/ZC3H12A antibody (A01688-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Sw620 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCPIP1/ZC3H12A antigen affinity purified polyclonal antibody (Catalog # A01688-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCPIP1/ZC3H12A at approximately 73KD. The expected band size for MCPIP1/ZC3H12A is at 66KD.
Click image to see more details
Figure 2. IHC analysis of MCPIP1/ZC3H12A using anti-MCPIP1/ZC3H12A antibody (A01688-2).
MCPIP1/ZC3H12A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rabbit anti-MCPIP1/ZC3H12A Antibody (A01688-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MCPIP1/ZC3H12A using anti-MCPIP1/ZC3H12A antibody (A01688-2).
MCPIP1/ZC3H12A was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rabbit anti-MCPIP1/ZC3H12A Antibody (A01688-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of MCPIP1/ZC3H12A using anti-MCPIP1/ZC3H12A antibody (A01688-2).
MCPIP1/ZC3H12A was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MCPIP1/ZC3H12A Antibody (A01688-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of U87 cells using anti-MCPIP1/ZC3H12A antibody (A01688-2).
Overlay histogram showing U87 cells stained with A01688-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCPIP1/ZC3H12A Antibody (A01688-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ZC3H12A (Source: Uniprot.org, NCBI)
Gene Name
ZC3H12A
Full Name
Endoribonuclease ZC3H12A
Weight
65.699kDa
Superfamily
ZC3H12 family
Alternative Names
MCPIP1; ZC3H12A; zinc finger CCCH-type containing 12A ZC3H12A MCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1 zinc finger CCCH-type containing 12A endoribonuclease ZC3H12A|MCP-1 treatment-induced protein|MCP-induced protein 1|bifunctional endoribonuclease and deubiquitinase ZC3H12A|monocyte chemotactic protein-induced protein 1|regnase-1|ribonuclease ZC3H12A|zinc finger CCCH domain-containing protein 12A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZC3H12A, check out the ZC3H12A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZC3H12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCPIP1/ZC3H12A Antibody Picoband™ (A01688-2)
Hello CJ!
No publications found for A01688-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MCPIP1/ZC3H12A Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-MCPIP1/ZC3H12A Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question