Anti-MDA5 IFIH1 Antibody (Monoclonal, 1B6)

Product Name

Anti-MDA5 IFIH1 Antibody (Monoclonal, 1B6)

View all MDA5 Antibodies

SKU/Catalog Number

M00263

Size

100μg/vial

Form

Liquid

Description

Boster Bio Anti-MDA5 IFIH1 Antibody (Monoclonal, 1B6) (Catalog# M00263). Tested in ELISA, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MDA5 IFIH1 Antibody (Monoclonal, 1B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00263)

Host

Mouse

Contents

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

1B6

Isotype

IgG1

Immunogen

Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects 32kDa. Does not cross-react with HO-2.

Reactive Species

M00263 is reactive to IFIH1 in Human, Mouse

Applications

M00263 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

116.689kDa

Background of MDA5

Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Western blot analysis of MDA5 monoclonal antibody (1B6): Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.

Gene/Protein Information For IFIH1 (Source: Uniprot.org, NCBI)

Alternative Names

CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; EC 3.6.4.13; helicard; Helicase with 2 CARD domains; Hlcd; IDDM19; interferon induced with helicase C domain 1; interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5MGC133047; MDA5RNA helicase-DEAD box protein 116; melanoma differentiation associated protein-5; Melanoma differentiation-associated protein 5; Murabutide down-regulated protein; RH116 IFIH1 AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1 interferon induced with helicase C domain 1 interferon-induced helicase C domain-containing protein 1|CADM-140 autoantigen|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RIG-I-like receptor 2|RNA helicase-DEAD box protein 116|clinically amyopathic dermatomyositis autoantigen 140 kDa|helicard|helicase with 2 CARD domains|melanoma differentiation-associated gene 5|melanoma differentiation-associated protein 5|murabutide down-regulated protein

For more info on IFIH1, check out the IFIH1 Infographic

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In this infographic, you will see the following information for IFIH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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