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Product Info Summary
| SKU: | A04803-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MDH2 Antibody (Center)
SKU/Catalog Number
A04803-1
Size
80 µl
Description
Boster Bio Anti-MDH2 Antibody (Center) (Catalog # A04803-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MDH2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04803-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the Central region of human MDH2.
Reactive Species
A04803-1 is reactive to MDH2 in Human, Mouse
Calculated molecular weight
35.5 kDa
Background of MDH2
MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04803-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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MDH2 Antibody (Center) western blot analysis in A431,Hela and mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the MDH2 antibody detected the MDH2 protein (arrow).
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-MDH2 Antibody (Center) (A04803-1)
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