Anti-MeCP2 Antibody

Boster Bio Anti-MeCP2 Antibody catalog # M00047-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: M00047-1
Size: 100ul
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-MeCP2 Antibody

See all MECP2 products

SKU/Catalog Number

M00047-1

Size

100ul

Description

Boster Bio Anti-MeCP2 Antibody catalog # M00047-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MeCP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00047-1)

Host

Rabbit

Contents

Affinity purified at 1mg/mL in PBS, 50% glycerol, 5mM NaN3

Clonality

Polyclonal

Immunogen

Full length recombinant human MeCP2

Reactive Species

M00047-1 is reactive to MECP2 in Human, Monkey, Mouse, Rat

Applications

M00047-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:10,000-1:20,000 IF/ICC or IHC: 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For MECP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P51608

Gene ID

4204

Gene Name

MECP2

Full Name

Methyl-CpG-binding protein 2

Weight

52.441kDa

Alternative Names

AUTSX3; DKFZp686A24160; MeCp-2 protein; mental retardation, X-linked 79; methyl CpG binding protein 2 (Rett syndrome); X-linked 16 MECP2 AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT methyl-CpG binding protein 2 methyl-CpG-binding protein 2|meCp-2 protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MECP2, check out the MECP2 Infographic

MECP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MECP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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17 Customer Q&As for Anti-MeCP2 Antibody

Question

I was wanting to use to test anti-MeCP2 antibody M00047-1 on human t-cell for research purposes, then I may be interested in using anti-MeCP2 antibody M00047-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

The products we sell, including anti-MeCP2 antibody M00047-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-24

Question

Is a blocking peptide available for product anti-MeCP2 antibody (M00047-1)?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

We do provide the blocking peptide for product anti-MeCP2 antibody (M00047-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-26

Question

Will M00047-1 anti-MeCP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

It shows on the product datasheet, M00047-1 anti-MeCP2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-27

Question

We have observed staining in rat cervix carcinoma erythroleukemia. What should we do? Is anti-MeCP2 antibody supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

From what I have seen in literature cervix carcinoma erythroleukemia does express MECP2. From what I have seen in Uniprot.org, MECP2 is expressed in cerebellar vermis, placenta, colon endothelium, skeletal muscle, cervix carcinoma, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MECP2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon endothelium, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Skeletal muscle, Pubmed ID: 8672133
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2019-08-05

Question

Have you ever done immunostaining with M00047-1?

Verified customer

Asked: 2019-08-01

Answer

Unfortunately, we have not done any in-house immunostaining with Anti-MeCP2 Antibody (M00047-1).

Boster Scientific Support

Answered: 2019-08-01

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-MeCP2 antibody M00047-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-02

Question

I have attached the WB image, lot number and protocol we used for t-cell using anti-MeCP2 antibody M00047-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-20

Question

We are currently using anti-MeCP2 antibody M00047-1 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

The anti-MeCP2 antibody (M00047-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-07

Question

Will anti-MeCP2 antibody M00047-1 work for IHC with t-cell?

M. Taylor

Verified customer

Asked: 2018-11-14

Answer

According to the expression profile of t-cell, MECP2 is highly expressed in t-cell. So, it is likely that anti-MeCP2 antibody M00047-1 will work for IHC with t-cell.

Boster Scientific Support

Answered: 2018-11-14

Question

We ordered your anti-MeCP2 antibody for WB on cervix carcinoma erythroleukemia last year. I am using rat, and We are going to use the antibody for IHC next. My lab would like examining cervix carcinoma erythroleukemia as well as skeletal muscle in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

M. Johnson

Verified customer

Asked: 2018-10-11

Answer

I took a look at the website and datasheets of our anti-MeCP2 antibody and it seems that M00047-1 has been tested on rat in both WB and IHC. Thus M00047-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-11

Question

Our team were content with the WB result of your anti-MeCP2 antibody. However we have observed positive staining in skeletal muscle nucleus. using this antibody. Is that expected? Could you tell me where is MECP2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-29

Answer

According to literature, skeletal muscle does express MECP2. Generally MECP2 expresses in nucleus. Regarding which tissues have MECP2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon endothelium, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Skeletal muscle, Pubmed ID: 8672133
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-06-29

Question

I was wanting to use your anti-MeCP2 antibody for IHC for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?

J. Lewis

Verified customer

Asked: 2018-05-15

Answer

As indicated on the product datasheet, M00047-1 anti-MeCP2 antibody has been tested for IHC, WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-15

Question

Is this M00047-1 anti-MeCP2 antibody reactive to the isotypes of MECP2?

Verified Customer

Verified customer

Asked: 2018-03-06

Answer

The immunogen of M00047-1 anti-MeCP2 antibody is Full length recombinant human MeCP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-03-06

Question

Is there a BSA free version of anti-MeCP2 antibody M00047-1 available?

T. Evans

Verified customer

Asked: 2017-07-21

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MeCP2 antibody M00047-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-21

Question

I see that the anti-MeCP2 antibody M00047-1 works with IHC, what is the protocol used to produce the result images on the product page?

J. Roberts

Verified customer

Asked: 2017-04-21

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-04-21

Question

My question regarding product M00047-1, anti-MeCP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Li

Verified customer

Asked: 2016-12-07

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00047-1 anti-MeCP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-12-07

Question

I am interested in using your anti-MeCP2 antibody for phosphatidylcholine metabolic process studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

L. Rodriguez

Verified customer

Asked: 2013-12-19

Answer

We appreciate your inquiry. This M00047-1 anti-MeCP2 antibody is tested on mouse brain, hela cells, c6 cells, neuronal cells. It is guaranteed to work for IHC, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-12-19

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M00047-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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