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Product Info Summary
| SKU: | A03859 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MED23/Crsp130 Antibody
SKU/Catalog Number
A03859
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MED23/Crsp130 Antibody (Catalog # A03859). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED23/Crsp130 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03859)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MED23.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03859 is reactive to MED23 in Human
Applications
A03859 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
156.474kDa
Background of MED23
Required for transcriptional activation subsequent to the assembly of the preinitiation complex By similarity. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
Wiemann S., Genome Res. 11:422-435(2001).
Mungall A.J., Nature 425:805-811(2003).
Akoulitchev S., Nature 407:102-106(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MED23 antibody A03859.
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Western blot analysis of extracts from A549 cells, using MED23 antiobdy A03859.
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Immunofluorescence analysis of A549 cells, using MED23 antibody A03859.
Protein Target Info & Infographic
Gene/Protein Information For MED23 (Source: Uniprot.org, NCBI)
Gene Name
MED23
Full Name
Mediator of RNA polymerase II transcription subunit 23
Weight
156.474kDa
Superfamily
Mediator complex subunit 23 family
Alternative Names
130 kDa transcriptional co-activator; 133 kDa transcriptional co-activator; CRSP130; DRIP130DKFZp434H0117; mediator complex subunit 23CRSP133; Protein sur-2 homolog; subunit 3, 130kDa; Transcriptional coactivator CRSP130; Vitamin D3 receptor-interacting protein complex 130 kDa component MED23 ARC130, CRSP130, CRSP133, CRSP3, DRIP130, MRT18, SUR-2, SUR2 mediator complex subunit 23 mediator of RNA polymerase II transcription subunit 23|130 kDa transcriptional co-activator|133 kDa transcriptional co-activator|activator-recruited cofactor 130 kDa component|cofactor required for Sp1 transcriptional activation subunit 3|mental retardation, non-syndromic, autosomal recessive, 18|vitamin D3 receptor interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED23, check out the MED23 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED23/Crsp130 Antibody (A03859)
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No publications found for A03859
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Customer Reviews
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