Product Info Summary
SKU: | M00292-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00292-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEK1/2 MAP2K1 Rabbit Monoclonal Antibody catalog # M00292-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00292-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BOA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEK1/2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00292-1 is reactive to MAP2K1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
3 kDa
Calculated molecular weight
43439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00292-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:100-1:500
ICC/IF 1:50-1:100
IP 1:50-1:100
Positive Control
WB: human 293T whole cell, human A549 whole cell, human A431 whole cell, human U2OS whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: human kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody.
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Immunofluorescent analysis of Hela cells, using MEK1/2 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K1 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K1
Full Name
Dual specificity mitogen-activated protein kinase kinase 1
Weight
43439 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;2.7.12.2;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K1;MEK1, PRKMK1; MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K1, check out the MAP2K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK1/2 MAP2K1 Rabbit Monoclonal Antibody (M00292-1)
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