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Product Info Summary
| SKU: | A02829 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-MEKKK 4 MAP4K4 Antibody
View all HGK/MAP4K4 Antibodies
SKU/Catalog Number
A02829
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MEKKK 4 MAP4K4 Antibody (Catalog # A02829). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKKK 4 MAP4K4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02829)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MEKKK 4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02829 is reactive to MAP4K4 in Human, Mouse
Applications
A02829 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
142.101kDa
Background of HGK/MAP4K4
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Yao Z., J. Biol. Chem. 274:2118-2125(1999).
Wright J.H., Mol. Cell. Biol. 23:2068-2082(2003).
Ishikawa K., DNA Res. 5:169-176(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells and HUVEC cells, using MEKKK 4 antibody A02829.
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Immunofluorescence analysis of A549 cells, using MEKKK 4 antibody A02829.
Protein Target Info & Infographic
Gene/Protein Information For MAP4K4 (Source: Uniprot.org, NCBI)
Gene Name
MAP4K4
Full Name
Mitogen-activated protein kinase kinase kinase kinase 4
Weight
142.101kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; FLH21957; FLJ10410; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HGKFLJ20373; HPK/GCK-like kinase HGK; KIAA0687; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; mitogen-activated protein kinase kinase kinase kinase 4; Nck-interacting kinase; NIKFLJ90111 MAP4K4 FLH21957, HEL-S-31, HGK, MEKKK4, NIK mitogen-activated protein kinase kinase kinase kinase 4 mitogen-activated protein kinase kinase kinase kinase 4|HPK/GCK-like kinase HGK|MAPK/ERK kinase kinase kinase 4|MEK kinase kinase 4|Ste20 group protein kinase HGK|epididymis secretory protein Li 31|hepatocyte progenitor kinase-like/germinal center kinase-like kinase|nck-interacting kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP4K4, check out the MAP4K4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP4K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEKKK 4 MAP4K4 Antibody (A02829)
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No publications found for A02829
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MEKKK 4 MAP4K4 Antibody
Question
Do you have a BSA free version of anti-MEKKK 4 antibody A02829 available?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MEKKK 4 antibody A02829 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-MEKKK 4 antibody A02829. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
I see that the anti-MEKKK 4 antibody A02829 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-21
Question
Here is the WB image, lot number and protocol we used for testis using anti-MEKKK 4 antibody A02829. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-09
Question
I have a question about product A02829, anti-MEKKK 4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02829 anti-MEKKK 4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-08
Question
We are currently using anti-MEKKK 4 antibody A02829 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
P. Anderson
Verified customer
Asked: 2016-09-01
Answer
The anti-MEKKK 4 antibody (A02829) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-01
Question
Would anti-MEKKK 4 antibody A02829 work on bovine IF with leukemic t-cell?
M. Jones
Verified customer
Asked: 2016-07-22
Answer
Our lab technicians have not tested anti-MEKKK 4 antibody A02829 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-MEKKK 4 antibody A02829 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine leukemic t-cell in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-22
