Anti-Melanocortin receptor 4 MC4R Antibody

Product Name

Anti-Melanocortin receptor 4 MC4R Antibody

View all MC4R Antibodies

SKU/Catalog Number

A00619

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Melanocortin receptor 4 MC4R Antibody (Catalog # A00619). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MC4R antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Melanocortin receptor 4 MC4R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00619)

Host

Rabbit

Contents

MC4R Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MC4R antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human MC4R. The immunogen is located within the first 50 amino acids of MC4R.

Reactive Species

A00619 is reactive to MC4R in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.9 kDa

Background of MC4R

The melanocortin-4 receptor (MC4R) is a member of the superfamily of seven transmembrane G-protein coupled receptors that are involved in multiple signal transduction pathways including the cAMP and MAPK signaling pathways. It is thought that the melanocortin system modulates energy expenditure and insulin sensitivity; activation of the MC4R results in the inhibition of c-Jun N-terminal kinase (JNK) activity and promotes insulin signaling. MC4R-null mice display maturity onset obesity characterized by hyperphagia, increased adiposity, hyperinsulinaemia and hyperleptinaemia, suggesting that like other obesity-linked genes such as FTO, PTER, and NPC1, MC4R is a potential candidate target for the treatment of obesity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00619 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MC4R antibody can be used for detection of MC4R by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MC4R

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