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Product Info Summary
| SKU: | A05037 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Melanoma-associated antigen D1 Maged1 Antibody
View all NRAGE/MAGED1 Antibodies
SKU/Catalog Number
A05037
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Melanoma-associated antigen D1 Maged1 Antibody (Catalog # A05037). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Melanoma-associated antigen D1 Maged1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05037)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05037 is reactive to MAGED1 in Human, Mouse, Rat
Applications
A05037 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
86.161kDa
Background of NRAGE/MAGED1
Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.
Zhao Z,et al.(2002) Sci China C Life Sci.45(4):412-20.
Shen WG,et al.(2007) Cell Commun Adhes.14(1):21-31.
Cheresh DA,et al. (1984) J Biol Chem.259(12):7453-9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 95kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
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Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 antibody A05037 and the same antibody preincubated with blocking peptide.
Protein Target Info & Infographic
Gene/Protein Information For MAGED1 (Source: Uniprot.org, NCBI)
Gene Name
MAGED1
Full Name
Melanoma-associated antigen D1
Weight
86.161kDa
Alternative Names
Dlxin-1; MAGE tumor antigen CCF; MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; Neurotrophin receptor-interacting MAGE homolog; NRAGE MAGE-D1 antigen; NRAGE MAGED1 DLXIN-1, NRAGE MAGE family member D1 melanoma-associated antigen D1|MAGE tumor antigen CCF|MAGE-D1 antigen|melanoma antigen family D, 1|melanoma antigen family D1|neurotrophin receptor-interacting MAGE homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAGED1, check out the MAGED1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAGED1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Melanoma-associated antigen D1 Maged1 Antibody (A05037)
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No publications found for A05037
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Melanoma-associated antigen D1 Maged1 Antibody
Question
Would anti-Melanoma-associated antigen D1 antibody A05037 work for WB with testis?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
According to the expression profile of testis, MAGED1 is highly expressed in testis. So, it is likely that anti-Melanoma-associated antigen D1 antibody A05037 will work for WB with testis.
Boster Scientific Support
Answered: 2020-04-10
Question
Does anti-Melanoma-associated antigen D1 antibody A05037 work on feline WB with fetal liver?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Our lab technicians have not tested anti-Melanoma-associated antigen D1 antibody A05037 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Melanoma-associated antigen D1 antibody A05037 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline fetal liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-03
Question
My question regards to test anti-Melanoma-associated antigen D1 antibody A05037 on rat testis for research purposes, then I may be interested in using anti-Melanoma-associated antigen D1 antibody A05037 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2020-01-06
Answer
The products we sell, including anti-Melanoma-associated antigen D1 antibody A05037, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-06
Question
Can you help my question with product A05037, anti-Melanoma-associated antigen D1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05037 anti-Melanoma-associated antigen D1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-05
Question
See attached the WB image, lot number and protocol we used for testis using anti-Melanoma-associated antigen D1 antibody A05037. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
Is this A05037 anti-Melanoma-associated antigen D1 antibody reactive to the isotypes of MAGED1?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The immunogen of A05037 anti-Melanoma-associated antigen D1 antibody is Peptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Melanoma-associated antigen D1 antibody A05037. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
Will A05037 anti-Melanoma-associated antigen D1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
You can see on the product datasheet, A05037 anti-Melanoma-associated antigen D1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-11
Question
Is a blocking peptide available for product anti-Melanoma-associated antigen D1 antibody (A05037)?
F. Carter
Verified customer
Asked: 2016-08-04
Answer
We do provide the blocking peptide for product anti-Melanoma-associated antigen D1 antibody (A05037). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-08-04
Question
I see that the anti-Melanoma-associated antigen D1 antibody A05037 works with WB, what is the protocol used to produce the result images on the product page?
A. Johnson
Verified customer
Asked: 2016-05-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-19
Question
I was wanting to use your anti-Melanoma-associated antigen D1 antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
R. Roberts
Verified customer
Asked: 2015-07-31
Answer
You can see on the product datasheet, A05037 anti-Melanoma-associated antigen D1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-31
Question
We are currently using anti-Melanoma-associated antigen D1 antibody A05037 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
A. Zhang
Verified customer
Asked: 2013-07-19
Answer
The anti-Melanoma-associated antigen D1 antibody (A05037) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-19
Question
Is there a BSA free version of anti-Melanoma-associated antigen D1 antibody A05037 available?
L. Krishna
Verified customer
Asked: 2013-01-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Melanoma-associated antigen D1 antibody A05037 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-02
