Anti-Galectin-1 / Human Placental Lactogen (hPL) Monoclonal Antibody

Product Name

Anti-Galectin-1 / Human Placental Lactogen (hPL) Monoclonal Antibody

View all Galectin-1 Antibodies

SKU/Catalog Number

M00470

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Galectin-1 / Human Placental Lactogen (hPL) Monoclonal Antibody (Catalog # M00470). Tested in WB, ELISA, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Galectin-1 / Human Placental Lactogen (hPL) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00470)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: GAL1/1831

Isotype

IgG1, lambda

Immunogen

Recombinant fragment (around aa12-108) of human Galectin-1 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00470 is reactive to LGALS1 in Human

Applications

M00470 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

14.716kDa

Background of Galectin-1

Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Anti-Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).

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Western Blot of HeLa, K562 and 293 cell lysates Anti-Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).

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Western Blot analysis of HeLa cell lysate using Anti-Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).

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Confocal immunofluorescence image of HeLa cells using Anti-Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831). Green (CF488) and Reddot is used to label the nuclei Red.

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Western Blot analysis of JEG-3 and K562 cell lysate using Anti-Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).

Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

For more info on LGALS1, check out the LGALS1 Infographic

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In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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