Anti-Metalloreductase STEAP3 STEAP3 Antibody

Product Name

Anti-Metalloreductase STEAP3 STEAP3 Antibody

View all STEAP3 Antibodies

SKU/Catalog Number

A05568-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Metalloreductase STEAP3 STEAP3 Antibody (Catalog # A05568-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

STEAP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Metalloreductase STEAP3 STEAP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05568-1)

Host

Rabbit

Contents

STEAP3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

STEAP3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human STEAP3. The immunogen is located within the first 50 amino acids of STEAP3.

Cross-reactivity

This STEAP3 antibody does not cross-react with other STEAP proteins.

Reactive Species

A05568-1 is reactive to STEAP3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.6 kDa

Background of STEAP3

The six-transmembrane epithelial antigen of prostate 3 (STEAP3) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 2 and STEAP4), STEAP3 promotes both iron and copper reduction. STEAP3 is highly expressed in hematopoietic tissues and colocalizes with the transferrin endosome. Overexpression of STEAP3 stimulates iron reduction; mice lacking STEAP3 are deficient in erythroid ferrireductase activity, suggesting that STEAP3 is an endosomal ferrireductase required for transferrin-dependent iron uptake in erythroid cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05568-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

STEAP3 antibody can be used for detection of STEAP3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information STEAP3

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