Product Info Summary
SKU: | M01065 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ (monoclonal, 2D6)
SKU/Catalog Number
M01065
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ (monoclonal, 2D6) catalog # M01065. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ (monoclonal, 2D6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01065)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2D6
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01065 is reactive to MMUT in Human, Mouse, Rat
Applications
M01065 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
83 kDa
Calculated molecular weight
83.134kDa
Background of MMUT
Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates;
Lane 2: human K562 whole cell lysates;
Lane 3: human HEK293 whole cell lysates;
Lane 4: human PC-3 whole cell lysates;
Lane 5: human Caco-2 whole cell lysates;
Lane 6: human Raji whole cell lysates;
Lane 7: rat PC-12 whole cell lysates;
Lane 8: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Methylmalonyl Coenzyme A antigen affinity purified monoclonal antibody (Catalog # M01065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Methylmalonyl Coenzyme A at approximately 83KD. The expected band size for Methylmalonyl Coenzyme A is at 83KD.
Click image to see more details
Figure 2. IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MMUT (Source: Uniprot.org, NCBI)
Gene Name
MMUT
Full Name
Methylmalonyl-CoA mutase, mitochondrial
Weight
83.134kDa
Superfamily
methylmalonyl-CoA mutase family
Alternative Names
Methylmalonyl-CoA mutase, mitochondrial MMUT MCM, MUT methylmalonyl-CoA mutase methylmalonyl-CoA mutase, mitochondrial|methylmalonyl Coenzyme A mutase|methylmalonyl-CoA isomerase|methylmalonyl-CoA mutase c.*192delA|methylmalonyl-CoA mutase c.*51C>G|methylmalonyl-CoA mutase variant c.1495G>A|methylmalonyl-CoA mutase variant c.2011A>G|methylmalonyl-CoA mutase variant c.2150G>T|methylmalonyl-CoA mutase variant c.322C>T|methylmalonyl-CoA mutase variant c.613_615delGAA|methylmalonyl-CoA mutase variant c.636G>A|methylmalonyl-CoA mutase variant c.643G>A|mutant methylmalonyl CoA mutase|truncated methylmalonyl CoA mutase|truncated methylmalonyl-CoA mutase variant c.1420C>T|truncated methylmalonyl-CoA mutase variant c.2179C>T|truncated methylmalonyl-CoA mutase variant c.91C>T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMUT, check out the MMUT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMUT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ (monoclonal, 2D6) (M01065)
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