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Product Info Summary
| SKU: | M07389 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-METTL14 Antibody (N-Term)
SKU/Catalog Number
M07389
Size
50 µl
Description
Boster Bio Anti-METTL14 Antibody (N-Term) (Catalog # M07389). Tested in IF, WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-METTL14 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07389)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This METTL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the human region of human METTL14.
Reactive Species
M07389 is reactive to METTL14 in Human, Mouse
Calculated molecular weight
52.2 kDa
Background of METTL14
N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and self-renewal of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing and processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding developmental regulators, results in transcript destabilization, maintaining the groung state of ESCs, thereby promoting self-renewal of ESCs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07389 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:250
Validation Images & Assay Conditions
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All lanes : Anti-METTL14 Antibody (N-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 52 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M07389 staining METTL14 in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-METTL14 Antibody (N-Term) (M07389)
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