Product Info Summary
SKU: | A10339 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MGAT4B Antibody Picoband™
SKU/Catalog Number
A10339
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MGAT4B Antibody Picoband™ catalog # A10339. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MGAT4B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10339)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MGAT4B recombinant protein (Position: Q31-D548).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10339 is reactive to MGAT4B in Human, Mouse, Rat
Applications
A10339 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
63.198kDa
Background of MGAT4B
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B is an enzyme that in humans is encoded by the MGAT4B gene. This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MGAT4B using anti-MGAT4B antibody (A10339).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGAT4B antigen affinity purified polyclonal antibody (Catalog # A10339) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGAT4B at approximately 70 kDa. The expected band size for MGAT4B is at 63 kDa.
Click image to see more details
Figure 2. IHC analysis of MGAT4B using anti-MGAT4B antibody (A10339).
MGAT4B was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MGAT4B Antibody (A10339) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MGAT4B (Source: Uniprot.org, NCBI)
Gene Name
MGAT4B
Full Name
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
Weight
63.198kDa
Superfamily
glycosyltransferase 54 family
Alternative Names
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B; alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; aminyltransferase; EC 2.4.1.145; GlcNAc-T IVb; GNT-IV; GNT-IVB; isoenzyme B; isozyme B; mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; N-acetylglucosaminyltransferase IVb; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb; UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IV; UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransfe MGAT4B GNT-IV, GNT-IVB alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B|N-acetylglucosaminyltransferase IVb|N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb|UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV|UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb|alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase|aminyltransferase|glcNAc-T IVb|mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isoenzyme B|mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MGAT4B, check out the MGAT4B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MGAT4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MGAT4B Antibody Picoband™ (A10339)
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