Anti-MGMT Antibody

Product Name

Anti-MGMT Antibody

View all MGMT Antibodies

SKU/Catalog Number

A01012-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MGMT Antibody catalog # A01012-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MGMT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01012-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MGMT

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01012-1 is reactive to MGMT in Human, Rat

Applications

A01012-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.646kDa

Background of MGMT

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-MGMT Antibody A01012-1

Western Blot (WB) analysis of specific cells using MGMT polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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Figure 2. Western blotting validation for Anti-MGMT Antibody A01012-1

Western Blot (WB) analysis of Jurkat cells using MGMT polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For MGMT (Source: Uniprot.org, NCBI)

Alternative Names

6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase MGMT O-6-methylguanine-DNA methyltransferase methylated-DNA--protein-cysteine methyltransferase|6-O-methylguanine-DNA methyltransferase|O-6-methylguanine-DNA-alkyltransferase|O6-methylguanine-DNA methyltransferase|methylguanine-DNA methyltransferase

For more info on MGMT, check out the MGMT Infographic

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In this infographic, you will see the following information for MGMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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