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Product Info Summary
| SKU: | A00466 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRAIL/Tnfsf10 Antibody Picoband®
SKU/Catalog Number
A00466
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAIL/Tnfsf10 Antibody Picoband® catalog # A00466. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAIL/Tnfsf10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00466)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TRAIL recombinant protein (Position: Y39-N291). Mouse TRAIL shares 66.3% amino acid (aa) sequence identity with human TRAIL.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00466 is reactive to Tnfsf10 in Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
33.5 kDa
Background of Tnfsf10
TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253). It is a member of Tumor Necrosis Factor Ligand Superfamily. In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL has got 281 amino acid proteins. TRAIL mRNA is expressed at significant levels in most normal tissues. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) and the receptors DcR1 and DcR2, leading to activation of specific kinases and transcription of genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00466 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: HEPA1-6 whole Cell, NIH3T3 whole Cell, PC12 whole Cell, NRK whole cell
Validation Images & Assay Conditions
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Western blot analysis of TRAIL using anti-TRAIL antibody (A00466).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEPA1-6 whole Cell lysates,
Lane 2: NIH3T3 whole Cell lysates,
Lane 3: PC12 whole Cell lysates,
Lane 4: NRK whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 20KD. The expected band size for TRAIL is at 35KD.
Specific Publications For Anti-TRAIL/Tnfsf10 Antibody Picoband® (A00466)
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