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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-TRAIL/Tnfsf10 Antibody Picoband™
Boster Bio Anti-TRAIL/Tnfsf10 Antibody Picoband™ catalog # A00466. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAIL/Tnfsf10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00466)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived mouse TRAIL recombinant protein (Position: Y39-N291). Mouse TRAIL shares 66.3% amino acid (aa) sequence identity with human TRAIL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00466 is reactive to Tnfsf10 in Mouse, Rat
A00466 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRAIL using anti-TRAIL antibody (A00466).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEPA1-6 whole Cell lysates,
Lane 2: NIH3T3 whole Cell lysates,
Lane 3: PC12 whole Cell lysates,
Lane 4: NRK whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 20KD. The expected band size for TRAIL is at 35KD.
Gene/Protein Information For Tnfsf10 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 10
tumor necrosis factor family
Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10 Tnfsf10|A330042I21Rik, AI448571, AP, APO-2L, Ly81, T, TL2, Tnlg6a, Trail|tumor necrosis factor (ligand) superfamily, member 10|tumor necrosis factor ligand superfamily member 10|TNF-related apoptosis-inducing ligand|TRAIL/APO2L|tumor necrosis factor ligand 6a*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfsf10, check out the Tnfsf10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfsf10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00466 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
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