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Product Info Summary
| SKU: | A01895 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MICU1/Cbara1 Monoclonal Antibody
SKU/Catalog Number
A01895
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MICU1/Cbara1 Monoclonal Antibody catalog # A01895. Tested in WB, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MICU1/Cbara1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01895)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
Mix
Isotype
IgG1
Immunogen
Recombinant Protein of MICU1
Reactive Species
A01895 is reactive to MICU1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
54.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01895 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-2000
IHC 1:100-200
IF 1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, MICU1 Monoclonal Antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). MICU1 Monoclonal Antibody (Mix) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Immunofluorescence analysis of Human-appendix tissue. 1, MICU1 Monoclonal Antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-MICU1/Cbara1 Monoclonal Antibody (A01895)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MICU1/Cbara1 Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle skin using anti-MICU1/Cbara1 Monoclonal antibody A01895. Let me know if you need anything else.
S. Li
Verified customer
Asked: 2020-03-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this A01895 anti-MICU1/Cbara1 Monoclonal antibody reactive to the isotypes of MICU1?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The immunogen of A01895 anti-MICU1/Cbara1 Monoclonal antibody is Recombinant Protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-14
Question
We are currently using anti-MICU1/Cbara1 Monoclonal antibody A01895 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-MICU1/Cbara1 Monoclonal antibody (A01895) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
Question
Is a blocking peptide available for product anti-MICU1/Cbara1 Monoclonal antibody (A01895)?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
We do provide the blocking peptide for product anti-MICU1/Cbara1 Monoclonal antibody (A01895). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-09-19
Question
I have a question about product A01895, anti-MICU1/Cbara1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01895 anti-MICU1/Cbara1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-30
Question
I see that the anti-MICU1/Cbara1 Monoclonal antibody A01895 works with WB, what is the protocol used to produce the result images on the product page?
S. Roberts
Verified customer
Asked: 2017-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-05
Question
I am looking for to test anti-MICU1/Cbara1 Monoclonal antibody A01895 on rat muscle skin for research purposes, then I may be interested in using anti-MICU1/Cbara1 Monoclonal antibody A01895 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Collins
Verified customer
Asked: 2013-12-12
Answer
The products we sell, including anti-MICU1/Cbara1 Monoclonal antibody A01895, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-12-12
