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Product Info Summary
| SKU: | M02518 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Milk Fat Globule (Breast Epithelial Marker) Monoclonal Antibody
SKU/Catalog Number
M02518
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Milk Fat Globule (Breast Epithelial Marker) Monoclonal Antibody (Catalog # M02518). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Milk Fat Globule (Breast Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02518)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: EDM45
Isotype
IgG1, kappa
Immunogen
Delipidated human milk fat globule membrane preparation
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02518 is reactive to MFGE8 in Human
Applications
M02518 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
43.105kDa
Background of MFG-E8
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Anti-Milk Fat Globule Monoclonal Antibody (EDM45)
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Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Anti-Milk Fat Globule Monoclonal Antibody (EDM45)
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Anti-Milk Fat Globule Monoclonal Antibody (EDM45)
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Formalin-fixed, paraffin-embedded human Pancreas stained with Anti-Milk Fat Globule Monoclonal Antibody (EDM45)
Protein Target Info & Infographic
Gene/Protein Information For MFGE8 (Source: Uniprot.org, NCBI)
Gene Name
MFGE8
Full Name
Lactadherin
Weight
43.105kDa
Alternative Names
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47 MFGE8 BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47 milk fat globule EGF and factor V/VIII domain containing lactadherin|O-acetyl disialoganglioside synthase|breast epithelial antigen BA46|medin|milk fat globule-EGF factor 8 protein|sperm associated antigen 10|sperm surface protein hP47
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MFGE8, check out the MFGE8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MFGE8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Milk Fat Globule (Breast Epithelial Marker) Monoclonal Antibody (M02518)
Hello CJ!
M02518 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants.
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