Anti-Mimitin NDUFAF2 Antibody

Product Name

Anti-Mimitin NDUFAF2 Antibody

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SKU/Catalog Number

A08718

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Mimitin NDUFAF2 Antibody (Catalog # A08718). Tested in ELISA, WB, IF applications. This antibody reacts with Human.

Storage & Handling

Mimitin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Mimitin NDUFAF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08718)

Host

Rabbit

Contents

Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin. The immunogen is located within the last 50 amino acids of Mimitin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

It has no cross responses to DAP or ZIP kinases. The approximately 70 kDa band is probably non-related to DRAK2 although it is peptide blockable.

Reactive Species

A08718 is reactive to Ndufaf2 in Human

Applications

A08718 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

19.628kDa

Background of Ndufaf2

Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.

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Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.

Gene/Protein Information For Ndufaf2 (Source: Uniprot.org, NCBI)

Alternative Names

B17.2LFLJ22398; B17.2-like; mimitin; MMTNmitochondrial; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein; NDUFA12-like Ndufaf2|1810058I14Rik, C86051, Ndufa, Ndufa12l, mim, mimitin|NADH:ubiquinone oxidoreductase complex assembly factor 2|NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2|MMTN|Myc-induced mitochondria protein|NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2|NDUFA12-like protein|mimitin, mitochondrial|myc-induced mitochondrial protein

For more info on Ndufaf2, check out the Ndufaf2 Infographic

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In this infographic, you will see the following information for Ndufaf2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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