Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated

Product Name

Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated

View all CCL20 Antibodies

SKU/Catalog Number

B00748-1

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated (Catalog # B00748-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store MIP-3 alpha Antibody Biotin Conjugated at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00748-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MIP-3 alpha Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3α protein.

Reactive Species

B00748-1 is reactive to CCL20 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

10.8 kDa

Background of CCL20

MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. MIP-1 alpha Antibody Biotin Conjugated is useful for researchers interested in Cancer, Cell Biology and Immunology Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

B00748-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
This purified antibody has been tested in western blotting and is suitable in ELISA. By western blot a band approximately 11 kDa in size corresponding to human MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information CCL20

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