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Product Info Summary
|Size:||For 5 plates, 96 wells each|
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Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0454)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A28-M97
7.8 pg/ml - 500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0454 is reactive to CCL20 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EZ0454 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ0454-CA||Rabbit anti- mouse Ccl20 polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0454-DA||Biotinylated goat anti- mouse Ccl20 polyclonal antibody (Detection Antibody)||500 µl, 3.3 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0454-ST||Lyophilized recombinant mouse Ccl20 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CCL20/MIP-3 alpha EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For CCL20 (Source: Uniprot.Org, NCBI)
C-C motif chemokine 20
intercrine beta (chemokine CC) family
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 Ccl20|CKb4, LARC, MI, MIP, MIP-3, MIP-3A, MIP-3[a], MIP3A, ST3, ST38, Scya, Scya20, exodus, exodus-1|chemokine (C-C motif) ligand 20|C-C motif chemokine 20|CC chemokine LARC|CC chemokine ST38|MIP-3-alpha|beta-chemokine exodus-1|liver and activation-regulated chemokine|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A20|small-inducible cytokine A20*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CCL20, check out the CCL20 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL20: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EZ0454 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li D, Wang A, Liu X, Meisgen F, Gr??nler J, Botusan Ir, Narayanan S, Erikci E, Li X, Blomqvist L, Du L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina Sb, St??hle M, Land??n Nx. J Clin Invest. 2015 Aug 3;125(8):3008-26. Doi: 10.1172/Jci79052. Epub 20...
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