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Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml. Cited in 1 publication(s).
|Size:||For 5 plates, 96 wells each|
|Sample Type:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Assay Range:||7.8 pg/ml - 500 pg/ml|
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See more CCL20/MIP-3 alpha products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml.|
|Cite This Product||Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0454)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
|Assay Range||7.8 pg/ml - 500 pg/ml|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: A28-M97|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each|
|NCBI Gene Id||20297|
|Species Of This Entry||Mouse|
|Protein Name||C-C motif chemokine 20|
|Superfamily||intercrine beta (chemokine CC) family|
|Alternative Names||CCL20/MIP-3 alpha|beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CCL20: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0454-CA||Rabbit anti- mouse Ccl20 polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0454-DA||Biotinylated goat anti- mouse Ccl20 polyclonal antibody (Detection Antibody)||500 µl, 3.3 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0454-ST||Lyophilized recombinant mouse Ccl20 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. 96 well microplate (Cat# AR1100)
6. Plate Sealers.
7. Capture Antibody Diluent: PBS.
8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered.
9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
11. Wash Buffer (PBS and PBS-T).
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered.
PBS-T: 0.1% Tween? 20 in PBS, pH 7.2-7.4.
*Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
This data is generated from a recent batch of EZ0454 Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-20minConcentration (pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.001|0.090|0.183|0.357|0.644|1.136|1.780|2.179
We measured random samples of Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean (pg/ml)|9|55|313|9|57|308 Standard deviation|0.62|3.02|21.28|0.78|3.7|21.56 CV (%)|6.9%|5.5%|6.8%|8.7%|6.5%|7%
We measured the performance consistency of Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Li D, Wang A, Liu X, Meisgen F, Gr??nler J, Botusan Ir, Narayanan S, Erikci E, Li X, Blomqvist L, Du L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina Sb, St??hle M, Land??n Nx. J Clin Invest. 2015 Aug 3;125(8):3008-26. Doi: 10.1172/Jci79052. Epub 20...||PubMed ID 26121747|
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