Product Info Summary
SKU: | M00733 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MMP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00733
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP1 Rabbit Monoclonal Antibody catalog # M00733. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00733)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CDG-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00733 is reactive to MMP1 in Human
Applications
M00733 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
54.007kDa
Background of MMP-1
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of MMP1 expression in MMP1 recombinant protein.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human hepatocellar carcinoma, using MMP1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MMP1 (Source: Uniprot.org, NCBI)
Gene Name
MMP1
Full Name
Interstitial collagenase
Weight
54.007kDa
Superfamily
peptidase M10A family
Alternative Names
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1 MMP1 CLG, CLGN matrix metallopeptidase 1 interstitial collagenase|fibroblast collagenase|matrix metallopeptidase 1 (interstitial collagenase)|matrix metalloprotease 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP1, check out the MMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP1 Rabbit Monoclonal Antibody (M00733)
Hello CJ!
M00733 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High plasma pentraxin 3 levels in diabetic polyneuropathy patients with nociceptive pain
Andrographolide plays an important role in bleomycin-induced pulmonary fibrosis treatment
Fan Hx, Chen Y, Ni Bx, Wang S, Sun M, Chen D, Zheng Jh. Onco Targets Ther. 2015 Jul 3;8:1619-26. Doi: 10.2147/Ott.S84561. Ecollection 2015. Expression Of Mmp-1/Par-1 And Patterns Of Invasion In Oral Squamous Cell Carcinoma As Potential Prognostic ...
Xu Y, Zhang Ja, Xu Y, Guo Sl, Wang S, Wu D, Wang Y, Luo D, Zhou Br. Stem Cells Dev. 2015 May 1;24(9):1096-111. Doi: 10.1089/Scd.2014.0321. Epub 2015 Jan 21. Antiphotoaging Effect Of Conditioned Medium Of Dedifferentiated Adipocytes On Skin In Vivo...
Deng Cl, Ling St, Liu Xq, Zhao Yj, Lv Yf. Exp Ther Med. 2015 Mar;9(3):992-998. Epub 2015 Jan 20. Decreased Expression Of Matrix Metalloproteinase-1 In The Maternal Umbilical Serum, Trophoblasts And Decidua Leads To Preeclampsia.
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-MMP1 Rabbit Monoclonal Antibody
Question
Does anti-MMP1 Rabbit Monoclonal antibody M00733 work for IF with synovial cell?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
According to the expression profile of synovial cell, MMP1 is highly expressed in synovial cell. So, it is likely that anti-MMP1 Rabbit Monoclonal antibody M00733 will work for IF with synovial cell.
Boster Scientific Support
Answered: 2020-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for synovial cell using anti-MMP1 Rabbit Monoclonal antibody M00733. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
My boss were happy with the WB result of your anti-MMP1 Rabbit Monoclonal antibody. However we have seen positive staining in ovary extracellular space using this antibody. Is that expected? Could you tell me where is MMP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Based on literature, ovary does express MMP1. Generally MMP1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP1 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2557822
Ovary, Pubmed ID: 15489334
Synovial cell, Pubmed ID: 3027129
Boster Scientific Support
Answered: 2019-11-20
Question
We need to test anti-MMP1 Rabbit Monoclonal antibody M00733 on human synovial cell for research purposes, then I may be interested in using anti-MMP1 Rabbit Monoclonal antibody M00733 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The products we sell, including anti-MMP1 Rabbit Monoclonal antibody M00733, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-21
Question
Is this M00733 anti-MMP1 Rabbit Monoclonal antibody reactive to the isotypes of MMP1?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The immunogen of M00733 anti-MMP1 Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-31
Question
We have seen staining in human synovial cell. Do you have any suggestions? Is anti-MMP1 Rabbit Monoclonal antibody supposed to stain synovial cell positively?
B. Kulkarni
Verified customer
Asked: 2019-07-12
Answer
Based on literature synovial cell does express MMP1. Based on Uniprot.org, MMP1 is expressed in smooth muscle tissue, ovary, synovial cell, fibroblast, among other tissues. Regarding which tissues have MMP1 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2557822
Ovary, Pubmed ID: 15489334
Synovial cell, Pubmed ID: 3027129
Boster Scientific Support
Answered: 2019-07-12
Question
Will M00733 anti-MMP1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Collins
Verified customer
Asked: 2019-06-27
Answer
As indicated on the product datasheet, M00733 anti-MMP1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-27
Question
We are currently using anti-MMP1 Rabbit Monoclonal antibody M00733 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-MMP1 Rabbit Monoclonal antibody (M00733) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
We ordered your anti-MMP1 Rabbit Monoclonal antibody for IF on fibroblast a few years ago. I am using human, and We want to use the antibody for WB next. My question regards examining fibroblast as well as ovary in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
I have checked the website and datasheets of our anti-MMP1 Rabbit Monoclonal antibody and I see that M00733 has been validated on human in both IF and WB. Thus M00733 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-14
Question
I have attached the WB image, lot number and protocol we used for synovial cell using anti-MMP1 Rabbit Monoclonal antibody M00733. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-06
Question
I was wanting to use your anti-MMP1 Rabbit Monoclonal antibody for IF for human synovial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human synovial cell identification?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
You can see on the product datasheet, M00733 anti-MMP1 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human synovial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-10
Question
Will anti-MMP1 Rabbit Monoclonal antibody M00733 work on feline WB with synovial cell?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
Our lab technicians have not validated anti-MMP1 Rabbit Monoclonal antibody M00733 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MMP1 Rabbit Monoclonal antibody M00733 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline synovial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-21
Question
Can you help my question with product M00733, anti-MMP1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00733 anti-MMP1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-06
Question
Is there a BSA free version of anti-MMP1 Rabbit Monoclonal antibody M00733 available?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP1 Rabbit Monoclonal antibody M00733 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-11
Question
I see that the anti-MMP1 Rabbit Monoclonal antibody M00733 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-17
Question
Is a blocking peptide available for product anti-MMP1 Rabbit Monoclonal antibody (M00733)?
J. Bhatt
Verified customer
Asked: 2014-10-23
Answer
We do provide the blocking peptide for product anti-MMP1 Rabbit Monoclonal antibody (M00733). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-10-23