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- Table of Contents
Pdx1 antibody (A00491-2) performs well in IF, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with A00491-2 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).
Designing a Western blot for PDX1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PDX1 Western Blot GuideBundle Save
Anti-(Mouse) Pdx1 Antibody (Center)
Pdx1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | IF, WB |
Recommend Dilution
Application
Dilution
Species
IF: 1:25
WB: 1:1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-(Mouse) Pdx1 Antibody (Center)
View all PDX1 Antibodies
View all PDX1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Pdx1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 136-169 amino acids from the Central region of Mouse Pdx1.
Cross-Reactivity
No cross reactivity with other proteins.
Calculated Molecular Weight
31.0 kDa
Background of Pdx1
Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
Cite This Product
Anti-(Mouse) Pdx1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00491-2)
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Product Promise
We promise Anti-(Mouse) Pdx1 Antibody (Center) performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00491-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 29, 2026