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Product Info Summary
| SKU: | A00491-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-(Mouse) Pdx1 Antibody (Center)
SKU/Catalog Number
A00491-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-(Mouse) Pdx1 Antibody (Center) (Catalog # A00491-2). Tested in WB, IF application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Pdx1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00491-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Pdx1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 136-169 amino acids from the Central region of Mouse Pdx1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00491-2 is reactive to Pdx1 in Mouse
Calculated molecular weight
31.0 kDa
Background of Pdx1
Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00491-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with A00491-2 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).
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Western blot analysis of lysate from mouse pancreas tissue lysate, using Pdx1 Antibody (Center) (Cat. #A00491-2). A00491-2 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Specific Publications For Anti-(Mouse) Pdx1 Antibody (Center) (A00491-2)
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