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- Table of Contents
Smad1 antibody (M00728-2) performs well in IHC-P, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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M00728-2 staining (Mouse) Smad1 in Mouse heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Designing a Western blot or IHC for SMAD1? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-(Mouse) Smad1 Antibody (Center)
Smad1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:1000
IHC-P: 1:25
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-(Mouse) Smad1 Antibody (Center)
View all SMAD1 Antibodies
View all SMAD1 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Smad1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-187 amino acids from the Central region of Mouse Smad1.
Calculated Molecular Weight
52.2 kDa
Background of Smad1
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene [removed]PubMed:15329343).
Cite This Product
Anti-(Mouse) Smad1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00728-2)
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Product Promise
We promise Anti-(Mouse) Smad1 Antibody (Center) performs excellently in relevant applications for IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00728-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026