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Product Info Summary
| SKU: | M01343-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-MPIP3 Antibody
SKU/Catalog Number
M01343-2
Size
50 µl
Description
Boster Bio Anti-MPIP3 Antibody (Catalog # M01343-2). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MPIP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01343-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1535CT627.6.80
Isotype
IgG2a,κ
Immunogen
This MPIP3 antibody is generated from a mouse immunized with a recombinant protein.
Reactive Species
M01343-2 is reactive to CDC25C in Human
Calculated molecular weight
53.4 kDa
Background of CDC25C
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01343-2 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-MPIP3 Antibody at 1:1000-1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: Raji whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01343-2 staining MPIP3 in human testis sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-MPIP3 Antibody (M01343-2)
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