Anti-MPZL1 Antibody Picoband™

Product Name

Anti-MPZL1 Antibody Picoband™

View all MPZL1 Antibodies

SKU/Catalog Number

A06667-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MPZL1 Antibody Picoband™ catalog # A06667-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MPZL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06667-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MPZL1 recombinant protein (Position: A37-N269).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06667-1 is reactive to MPZL1 in Human

Applications

A06667-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

29-37 kDa

Calculated molecular weight

29.082kDa

Background of MPZL1

Myelin protein zero-like protein 1 is a protein that in humans is encoded by the MPZL1 gene. MPZL1 is a cell surface receptor, which is involved in signal transduction processes. MPZL1 recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. MPZL1 is a major receptor for concanavalin A (ConA) and is involved in cell.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MPZL1 using anti-MPZL1 antibody (A06667-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPZL1 antigen affinity purified polyclonal antibody (Catalog # A06667-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPZL1 at approximately 29-37 kDa. The expected band size for MPZL1 is at 29 kDa.

Gene/Protein Information For MPZL1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ21047; immunoglobulin family transmembrane protein; MPZL1b; myelin protein zero-like 1; myelin protein zero-like protein 1; protein zero related; Protein zero-related; PZR1b; PZRb; PZRPZRa MPZL1 MPZL1b, PZR, PZR1b, PZRa, PZRb myelin protein zero like 1 myelin protein zero-like protein 1|immunoglobulin family transmembrane protein|protein zero related

For more info on MPZL1, check out the MPZL1 Infographic

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In this infographic, you will see the following information for MPZL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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