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Product Info Summary
| SKU: | A14134-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MRP-L32 Antibody
SKU/Catalog Number
A14134-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MRP-L32 Antibody catalog # A14134-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-L32 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14134-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MRP-L32
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14134-2 is reactive to MRPL32 in Human
Applications
A14134-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.405kDa
Background of MRPL32
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.
Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Kenmochi N., Genomics 77:65-70(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Figure 1. Immunohistochemistry validation of MRPL32 using Anti-MRP-L32 Antibody (A14134-2).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CProtein Target Info & Infographic
Gene/Protein Information For MRPL32 (Source: Uniprot.org, NCBI)
Gene Name
MRPL32
Full Name
39S ribosomal protein L32, mitochondrial
Weight
21.405kDa
Superfamily
bacterial ribosomal protein bL32 family
Alternative Names
39S ribosomal protein L32, mitochondrial; HSPC283; L32mtMRP-L32bMRP-59b; mitochondrial ribosomal protein L32 MRPL32 HSPC283, L32mt, MRP-L32, bMRP-59b mitochondrial ribosomal protein L32 39S ribosomal protein L32, mitochondrial|mitochondrial large ribosomal subunit protein bL32m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL32, check out the MRPL32 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-L32 Antibody (A14134-2)
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No publications found for A14134-2
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