Product Info Summary
SKU: | A14506 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MRP-L35 Antibody
View all mitochondrial ribosomal protein L35 Antibodies
SKU/Catalog Number
A14506
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-L35 Antibody catalog # A14506. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-L35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14506)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human MRP-L35. at AA rangle: 80-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14506 is reactive to MRPL35 in Human, Mouse
Applications
A14506 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.514kDa
Background of mitochondrial ribosomal protein L35
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 293T COLO205 using MRP-L35 antibody.
Click image to see more details
Western Blot (WB) analysis of 293 using MRP-L35 antibody.
Protein Target Info & Infographic
Gene/Protein Information For MRPL35 (Source: Uniprot.org, NCBI)
Gene Name
MRPL35
Full Name
39S ribosomal protein L35, mitochondrial
Weight
21.514kDa
Superfamily
bacterial ribosomal protein bL35 family
Alternative Names
L35mt; mitochondrial ribosomal protein L35; MRP-L35,39S ribosomal protein L35, mitochondrial MRPL35 L35mt, MRP-L35 mitochondrial ribosomal protein L35 39S ribosomal protein L35, mitochondrial|mitochondrial large ribosomal subunit protein bL35m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL35, check out the MRPL35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-L35 Antibody (A14506)
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No publications found for A14506
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-MRP-L35 Antibody
Question
We are currently using anti-MRP-L35 antibody A14506 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The anti-MRP-L35 antibody (A14506) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-28
Question
Is this A14506 anti-MRP-L35 antibody reactive to the isotypes of MRPL35?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The immunogen of A14506 anti-MRP-L35 antibody is Synthesized peptide derived from the Internal region of human MRP-L35. at AA rangle: 80-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach using anti-MRP-L35 antibody A14506. Let me know if you need anything else.
K. Roberts
Verified customer
Asked: 2018-02-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-22
Question
Can you help my question with product A14506, anti-MRP-L35 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14506 anti-MRP-L35 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-01