Anti-MRP-L35 Antibody

mitochondrial ribosomal protein L35 antibody

Boster Bio Anti-MRP-L35 Antibody catalog # A14506. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A14506
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-MRP-L35 Antibody

View all mitochondrial ribosomal protein L35 Antibodies

SKU/Catalog Number

A14506

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MRP-L35 Antibody catalog # A14506. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRP-L35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14506)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human MRP-L35. at AA rangle: 80-160

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14506 is reactive to MRPL35 in Human, Mouse

Applications

A14506 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.514kDa

Background of mitochondrial ribosomal protein L35

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MRPL35 (Source: Uniprot.org, NCBI)

Gene Name

MRPL35

Full Name

39S ribosomal protein L35, mitochondrial

Weight

21.514kDa

Superfamily

bacterial ribosomal protein bL35 family

Alternative Names

L35mt; mitochondrial ribosomal protein L35; MRP-L35,39S ribosomal protein L35, mitochondrial MRPL35 L35mt, MRP-L35 mitochondrial ribosomal protein L35 39S ribosomal protein L35, mitochondrial|mitochondrial large ribosomal subunit protein bL35m

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MRPL35, check out the MRPL35 Infographic

MRPL35 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRPL35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-MRP-L35 Antibody

Question

We are currently using anti-MRP-L35 antibody A14506 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

The anti-MRP-L35 antibody (A14506) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-28

Question

Is this A14506 anti-MRP-L35 antibody reactive to the isotypes of MRPL35?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

The immunogen of A14506 anti-MRP-L35 antibody is Synthesized peptide derived from the Internal region of human MRP-L35. at AA rangle: 80-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-21

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach using anti-MRP-L35 antibody A14506. Let me know if you need anything else.

K. Roberts

Verified customer

Asked: 2018-02-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-22

Question

Can you help my question with product A14506, anti-MRP-L35 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-11-01

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14506 anti-MRP-L35 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-11-01

Order DetailsPrice
A14506

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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