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Product Info Summary
| SKU: | A14945 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MRP-S34 Antibody
SKU/Catalog Number
A14945
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-S34 Antibody catalog # A14945. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14945)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS34. AA range:169-218
Reactive Species
A14945 is reactive to MRPS34 in Human, Mouse
Calculated molecular weight
25.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14945 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of Jurkat cells using MRP-S34 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HeLa, HUVEC, HT-29, A549, and Jurkat cells, using MRPS34 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MRP-S34 Antibody (A14945)
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Customer Q&As
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6 Customer Q&As for Anti-MRP-S34 Antibody
Question
We need to test anti-MRP-S34 antibody A14945 on human eye for research purposes, then I may be interested in using anti-MRP-S34 antibody A14945 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The products we sell, including anti-MRP-S34 antibody A14945, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-22
Question
Would anti-MRP-S34 antibody A14945 work on canine WB with mucosa of transverse colon?
J. Brown
Verified customer
Asked: 2019-08-21
Answer
Our lab technicians have not validated anti-MRP-S34 antibody A14945 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MRP-S34 antibody A14945 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine mucosa of transverse colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-21
Question
Is a blocking peptide available for product anti-MRP-S34 antibody (A14945)?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
We do provide the blocking peptide for product anti-MRP-S34 antibody (A14945). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-08
Question
We are currently using anti-MRP-S34 antibody A14945 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
The anti-MRP-S34 antibody (A14945) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-03
Question
Does A14945 anti-MRP-S34 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Wu
Verified customer
Asked: 2017-07-14
Answer
You can see on the product datasheet, A14945 anti-MRP-S34 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-14
Question
I have a question about product A14945, anti-MRP-S34 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Williams
Verified customer
Asked: 2014-12-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14945 anti-MRP-S34 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-30
