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Product Info Summary
| SKU: | A14072S9 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MRP-S9 (F132) Antibody
SKU/Catalog Number
A14072S9
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MRP-S9 (F132) Antibody catalog # A14072S9. Tested in WB,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S9 (F132) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14072S9)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPS9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14072S9 is reactive to MRPS9 in Human
Applications
A14072S9 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
45.835kDa
Background of MRPS9
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Koc E.C., J. Biol. Chem. 276:19363-19374(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of MRPS9 using Anti-MRP-S9 (F132) Antibody (A14072S9).
Immunohistochemistry (IHC) analyzes of MRP-S9 (F132) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-MRP-S9 (F132) Antibody A14072S9
Western blot (WB) analysis of MRP-S9 (F132) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MRPS9 (Source: Uniprot.org, NCBI)
Gene Name
MRPS9
Full Name
28S ribosomal protein S9, mitochondrial
Weight
45.835kDa
Superfamily
universal ribosomal protein uS9 family
Alternative Names
mitochondrial ribosomal protein S9; MRP-S9RPMS9S9mt28S ribosomal protein S9, mitochondrial MRPS9 MRP-S9, RPMS9, S9mt mitochondrial ribosomal protein S9 28S ribosomal protein S9, mitochondrial|mitochondrial small ribosomal subunit protein uS9m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPS9, check out the MRPS9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-S9 (F132) Antibody (A14072S9)
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No publications found for A14072S9
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