Click image to see more details
Product Info Summary
| SKU: | M00974 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-MRP2 ABCC2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00974
BM5126 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MRP2 ABCC2 Rabbit Monoclonal Antibody catalog # M00974. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP2 ABCC2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00974)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABDD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MRP2
Reactive Species
M00974 is reactive to ABCC2 in Human
Observed Molecular Weight
250 kDa
Calculated molecular weight
174.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00974 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human HepG2 whole cell, human A549 whole cell, human CACO-2 whole cell, human HUH-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ABCC2 using anti-ABCC2 antibody (M00974).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HUH-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC2 antigen affinity purified monoclonal antibody (Catalog # M00974) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCC2 at approximately 250 kDa. The expected band size for ABCC2 is at 174 kDa.
Specific Publications For Anti-MRP2 ABCC2 Rabbit Monoclonal Antibody (M00974)
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Customer Q&As
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5 Customer Q&As for Anti-MRP2 ABCC2 Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-MRP2 Rabbit Monoclonal antibody M00974 available?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MRP2 Rabbit Monoclonal antibody M00974 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-11
Question
I was wanting to use your anti-MRP2 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
You can see on the product datasheet, M00974 anti-MRP2 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-MRP2 Rabbit Monoclonal antibody M00974. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Would M00974 anti-MRP2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Zhao
Verified customer
Asked: 2019-06-19
Answer
As indicated on the product datasheet, M00974 anti-MRP2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-MRP2 Rabbit Monoclonal antibody M00974. Let me know if you need anything else.
J. Carter
Verified customer
Asked: 2015-09-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-30
