Click image to see more details
Product Info Summary
| SKU: | A10301 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MRPL39 Antibody
SKU/Catalog Number
A10301
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL39 Antibody (Catalog # A10301). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL39 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10301)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPL39.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10301 is reactive to Mrpl39 in Human, Mouse
Applications
A10301 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.549kDa
Background of Mrpl39
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been described. A pseudogene corresponding to this gene is found on chromosome 5q.
Choi D.K., Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Spirina O., Gene 261:229-234(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from MCF-7 cells and Jurkat cells, using MRPL39 antibody A10301.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human heart tissue using MRPL39 antibody A10301.
Protein Target Info & Infographic
Gene/Protein Information For Mrpl39 (Source: Uniprot.org, NCBI)
Gene Name
Mrpl39
Full Name
39S ribosomal protein L39, mitochondrial
Weight
38.549kDa
Superfamily
mitochondrion-specific ribosomal protein mL39 family
Alternative Names
C21orf92PRED66; FLJ20451,39S ribosomal protein L39, mitochondrial; L39mt39S ribosomal protein L5, mitochondrial; MGC104174; MGC3400; mitochondrial ribosomal protein L39; MRP-L5L5mt; MRPL5MSTP003; PRED22; RPML5MRP-L39
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mrpl39, check out the Mrpl39 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mrpl39: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL39 Antibody (A10301)
Hello CJ!
No publications found for A10301
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MRPL39 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-MRPL39 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-MRPL39 Antibody
Question
We are currently using anti-MRPL39 antibody A10301 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-MRPL39 antibody (A10301) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
Please see the WB image, lot number and protocol we used for testis using anti-MRPL39 antibody A10301. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
I have a question about product A10301, anti-MRPL39 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10301 anti-MRPL39 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-09
