Anti-MRPL4 Antibody

Product Name

Anti-MRPL4 Antibody

View all mitochondrial ribosomal protein L4 Antibodies

SKU/Catalog Number

A14430

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPL4 Antibody (Catalog # A14430). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14430)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPL4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14430 is reactive to MRPL4 in Human, Mouse

Applications

A14430 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

34.919kDa

Background of mitochondrial ribosomal protein L4

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms.

Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
Lai C.-H., Genome Res. 10:703-713(2000).
Grimwood J., Nature 428:529-535(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from COLO cells, using MRPL4 antibody A14430.

Gene/Protein Information For MRPL4 (Source: Uniprot.org, NCBI)

Alternative Names

CGI-28; L4mt; MGC16367; MGC2681; mitochondrial ribosomal protein L4,39S ribosomal protein L4, mitochondrial; MRP-L4 MRPL4 CGI-28, L4mt mitochondrial ribosomal protein L4 39S ribosomal protein L4, mitochondrial|MRP-L4|mitochondrial large ribosomal subunit protein uL4m

For more info on MRPL4, check out the MRPL4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRPL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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