Anti-MRPL50 Antibody

Product Name

Anti-MRPL50 Antibody

View all Mrpl50 Antibodies

SKU/Catalog Number

A17223

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPL50 Antibody (Catalog # A17223). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPL50 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17223)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPL50.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A17223 is reactive to Mrpl50 in Human

Applications

A17223 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

18.213kDa

Background of Mrpl50

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.

Ota T., Nat. Genet. 36:40-45(2004).
Humphray S.J., Nature 429:369-374(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from COLO cells and HT-29 cells, using MRPL50 antibody A17223.

Gene/Protein Information For Mrpl50 (Source: Uniprot.org, NCBI)

Alternative Names

39S ribosomal protein L50, mitochondrial; FLJ20493; L50mt; mitochondrial 39S ribosomal protein L50; mitochondrial ribosomal protein L50; MRP-L50FLJ21990

For more info on Mrpl50, check out the Mrpl50 Infographic

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In this infographic, you will see the following information for Mrpl50: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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