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Product Info Summary
| SKU: | MA1040 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-VEGFR2/KDR Monoclonal Antibody
SKU/Catalog Number
MA1040
BM0864 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGFR2/KDR Monoclonal Antibody catalog # MA1040. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGFR2/KDR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1040)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
KDR-1
Isotype
Mouse IgG1
Immunogen
Recombinant human extracellular domain of VEGFR-2 (KDR).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1040 is reactive to Kdr in Human
Calculated molecular weight
150.4 kDa
Background of Kdr
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The importance of VEGF and its receptor system in tumor growth and intervention in this system may provide promising approaches to cancer therapy. VEGF receptor 2 is a member of a receptor tyrosine kinase family. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1040 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For Anti-VEGFR2/KDR Monoclonal Antibody (MA1040)
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Customer Q&As
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5 Customer Q&As for Anti-VEGFR2/KDR Monoclonal Antibody
Question
Our lab were content with the WB result of your anti-VEGFR2/KDR Monoclonal antibody. However we have observed positive staining in umbilical vein cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
According to literature, umbilical vein does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-VEGFR2/KDR Monoclonal antibody MA1040 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-VEGFR2/KDR Monoclonal antibody (MA1040) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
Question
What is the recommended dilution protocol of MA1040?
Verified customer
Asked: 2019-10-21
Answer
The recommended dilution of the Anti-VEGFR2/KDR Monoclonal Antibody (MA1040): Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml. Immunohistochemistry(Frozen Section), 1-2μg/ml, Human
Boster Scientific Support
Answered: 2019-10-21
Question
Were you able to test your VEGFR2 antibody against a rabbit VEGFR2 protein?
Verified customer
Asked: 2019-10-17
Answer
We have not tested our Anti-VEGFR2/KDR Monoclonal Antibody MA1040 antibody against a rabbit VEGFR2 protein, so we cannot guarantee that this would work.
Boster Scientific Support
Answered: 2019-10-17
Question
We have seen staining in human lung. Do you have any suggestions? Is anti-VEGFR2/KDR Monoclonal antibody supposed to stain lung positively?
J. Johnson
Verified customer
Asked: 2015-07-13
Answer
According to literature lung does express KDR. According to Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2015-07-13
