Anti-VEGFR2/KDR Monoclonal Antibody

Boster Bio Anti-VEGFR2/KDR Monoclonal Antibody catalog # MA1040. Tested in IHC applications. This antibody reacts with Human. Cited in 8 publication(s).

Product Info Summary

SKU: MA1040
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC

Product Name

Anti-VEGFR2/KDR Monoclonal Antibody

See all VEGFR2/KDR/Flk-1 products

SKU/Catalog Number

MA1040

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGFR2/KDR Monoclonal Antibody catalog # MA1040. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGFR2/KDR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1040)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

KDR-1

Isotype

Mouse IgG1

Immunogen

Recombinant human extracellular domain of VEGFR-2 (KDR).

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1040 is reactive to Kdr in Human

Applications

MA1040 is guaranteed for IHC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 1-2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Kdr (Source: Uniprot.Org, NCBI)

Uniprot ID

O08775

Gene ID

25589

Gene Name

Kdr

Full Name

Vascular endothelial growth factor receptor 2

Weight

150.394kDa

Superfamily

protein kinase superfamily

Alternative Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 Kdr|Vegfr-2|kinase insert domain receptor|vascular endothelial growth factor receptor 2|FLK-1|FLK1 kinase insert domain receptor (VEGF receptor 2)|FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2)|fetal liver kinase 1|kinase insert domain protein receptor|protein-tyrosine kinase receptor flk-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Kdr, check out the Kdr Infographic

Kdr infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Kdr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1040 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang L,Zhang J,Gong Y,Lv L.Systematic and experimental investigations of the anti-colorectal cancer mediated by genistein. Biofactors.2020 Nov;46(6):974-982.doi:10.1002/biof.1677.Epub 2020 Sep 20.PMID:32951326.
Species: Human,Mouse
MA1040 usage in article: APP:IF, SAMPLE:CRC TISSUE, DILUTION:1:100

Zhou D, Zhan S, Zhou D, Li Z, Lin X, Tang K, Shu H, Chen G, Zeng S, Cai Y, Jiang X. Cell Mol Neurobiol. 2011 Jul;31(5):687-94. Doi: 10.1007/S10571-011-9665-6. Epub 2011 Mar 9. A Study Of The Distribution And Density Of The Vegfr-2 Receptor On Glio...

Li Ql, Huang N, Chen C, Chen Jl, Xiong Kq, Chen Jy, You Tx, Jin J, Liang X. J Biomed Mater Res A. 2010 Sep 15;94(4):1283-93. Doi: 10.1002/Jbm.A.32812. Oriented Immobilization Of Anti-Cd34 Antibody On Titanium Surface For Self-Endothelialization In...

Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...

Lin J, Li G, Den X, Xu C, Liu S, Gao Y, Liu H, Zhang J, Li X, Liang S. Brain Res Bull. 2010 Oct 30;83(5):284-91. Doi: 10.1016/J.Brainresbull.2010.08.002. Epub 2010 Aug 10. Vegf And Its Receptor-2 Involved In Neuropathic Pain Transmission Mediated ...

Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1

Ding G, Chen X, Zhu J, Feng Z. Cytotechnology. 2014 May;66(3):395-411. Doi: 10.1007/S10616-013-9587-X. Epub 2013 Jul 24. A Murine-Human Chimeric Igg Antibody Against Vascular Endothelial Growth Factor Receptor 2 Inhibits Angiogenesis In Vitro.

Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.

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5 Customer Q&As for Anti-VEGFR2/KDR Monoclonal Antibody

Question

Our lab were content with the WB result of your anti-VEGFR2/KDR Monoclonal antibody. However we have observed positive staining in umbilical vein cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

According to literature, umbilical vein does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2020-02-24

Question

We are currently using anti-VEGFR2/KDR Monoclonal antibody MA1040 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

The anti-VEGFR2/KDR Monoclonal antibody (MA1040) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the recommended dilution protocol of MA1040?

Verified customer

Asked: 2019-10-21

Answer

The recommended dilution of the Anti-VEGFR2/KDR Monoclonal Antibody (MA1040): Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml. Immunohistochemistry(Frozen Section), 1-2μg/ml, Human

Boster Scientific Support

Answered: 2019-10-21

Question

Were you able to test your VEGFR2 antibody against a rabbit VEGFR2 protein?

Verified customer

Asked: 2019-10-17

Answer

We have not tested our Anti-VEGFR2/KDR Monoclonal Antibody MA1040 antibody against a rabbit VEGFR2 protein, so we cannot guarantee that this would work.

Boster Scientific Support

Answered: 2019-10-17

Question

We have seen staining in human lung. Do you have any suggestions? Is anti-VEGFR2/KDR Monoclonal antibody supposed to stain lung positively?

J. Johnson

Verified customer

Asked: 2015-07-13

Answer

According to literature lung does express KDR. According to Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2015-07-13

Size

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MA1040
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