Anti-VEGFR2/KDR Monoclonal Antibody


SKU MA1040
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number KDR-1
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC

Overview

Product Name Anti-VEGFR2/KDR Monoclonal Antibody
SKU/Catalog Number MA1040
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for VEGFR2, kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR) detection. Tested with IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-VEGFR2/KDR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1040)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Recombinant human extracellular domain of VEGFR-2(KDR).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Images And Assay Conditions

Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O08775
Gene Name Kdr
Protein Name Vascular endothelial growth factor receptor 2
Tissue Specificity Expressed in the post-pubertal mammary glands. .
Alternative Names Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Protein-tyrosine kinase receptor flk-1;CD309;Kdr;Flk1;
Subcellular Localization Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Nucleus . Cytoplasmic vesicle . Early endosome . Cell junction . Endoplasmic reticulum . Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus. Localized with RAP1A at cell-cell junctions. Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (By similarity). .
Molecular Weight 150394 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5- trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA- mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC (By similarity). .
Research Areas Human

*You can search these to find other products in these research areas.
Background VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The importance of VEGF and its receptor system in tumor growth and intervention in this system may provide promising approaches to cancer therapy. VEGF receptor 2 is a member of a receptor tyrosine kinase family. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues.

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Monoclonal clone# G2 antibody for FLK 1/KDR detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. FLK 1/KDR information: Molecular Weight: 150394 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Nucleus . Cytoplasmic vesicle . Early endosome . Cell junction . Endoplasmic reticulum . Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus. Localized with RAP1A at cell-cell junctions. Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (By similarity); Tissue Specificity: Expressed in the post-pubertal mammary glands.
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In stock
Order Product
MA1040
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Zhou D, Zhan S, Zhou D, Li Z, Lin X, Tang K, Shu H, Chen G, Zeng S, Cai Y, Jiang X. Cell Mol Neurobiol. 2011 Jul;31(5):687-94. Doi: 10.1007/S10571-011-9665-6. Epub 2011 Mar 9. A Study Of The Distribution And Density Of The Vegfr-2 Receptor On Glio...
Li Ql, Huang N, Chen C, Chen Jl, Xiong Kq, Chen Jy, You Tx, Jin J, Liang X. J Biomed Mater Res A. 2010 Sep 15;94(4):1283-93. Doi: 10.1002/Jbm.A.32812. Oriented Immobilization Of Anti-Cd34 Antibody On Titanium Surface For Self-Endothelialization In...
Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...
Lin J, Li G, Den X, Xu C, Liu S, Gao Y, Liu H, Zhang J, Li X, Liang S. Brain Res Bull. 2010 Oct 30;83(5):284-91. Doi: 10.1016/J.Brainresbull.2010.08.002. Epub 2010 Aug 10. Vegf And Its Receptor-2 Involved In Neuropathic Pain Transmission Mediated ...
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Ding G, Chen X, Zhu J, Feng Z. Cytotechnology. 2014 May;66(3):395-411. Doi: 10.1007/S10616-013-9587-X. Epub 2013 Jul 24. A Murine-Human Chimeric Igg Antibody Against Vascular Endothelial Growth Factor Receptor 2 Inhibits Angiogenesis In Vitro.
Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to vegfr2 antibody, flk1 antibody, flk-1 antibody, kdr antibody, vegfr 2 antibody, vegfr antibody, vegfr-2 antibody
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