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Product Info Summary
| SKU: | A14080 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MRPS25 Antibody
SKU/Catalog Number
A14080
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPS25 Antibody (Catalog # A14080). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPS25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14080)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPS25.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14080 is reactive to MRPS25 in Human, Mouse
Applications
A14080 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
20.116kDa
Background of MRPS25
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.
Ota T., Nat. Genet. 36-40-45(2004).
Hattori A., DNA Res. 7:357-366(2000).
Koc E.C., J. Biol. Chem. 275:32585-32591(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using MRPS25 antibody A14080.
Protein Target Info & Infographic
Gene/Protein Information For MRPS25 (Source: Uniprot.org, NCBI)
Gene Name
MRPS25
Full Name
28S ribosomal protein S25, mitochondrial
Weight
20.116kDa
Superfamily
mitochondrion-specific ribosomal protein mS25 family
Alternative Names
FLJ00023; mitochondrial 28S ribosomal protein S25; mitochondrial ribosomal protein S25; MRP-S2528S ribosomal protein S25, mitochondrial; RPMS25DKFZp313H0817; S25mt MRPS25 COXPD50, MRP-S25, RPMS25 mitochondrial ribosomal protein S25 28S ribosomal protein S25, mitochondrial|S25mt|mitochondrial 28S ribosomal protein S25|mitochondrial small ribosomal subunit protein mS25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPS25, check out the MRPS25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPS25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPS25 Antibody (A14080)
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No publications found for A14080
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