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Product Info Summary
| SKU: | M01203 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-mSin3A Rabbit Monoclonal Antibody
SKU/Catalog Number
M01203
BM5270 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-mSin3A Rabbit Monoclonal Antibody catalog # M01203. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mSin3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01203)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACEE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human mSin3A Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
Reactive Species
M01203 is reactive to SIN3A in Human, Mouse, Rat
Observed Molecular Weight
150 kDa
Calculated molecular weight
145.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01203 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: K562 cell, RAW 2647 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of mSin3A expression in (1) K562 cell lysate; (2) RAW 264.7 cell lysate.
Specific Publications For Anti-mSin3A Rabbit Monoclonal Antibody (M01203)
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Customer Q&As
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6 Customer Q&As for Anti-mSin3A Rabbit Monoclonal Antibody
Question
Would anti-mSin3A Monoclonal antibody M01203 work for WB with brain teratocarcinoma?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to the expression profile of brain teratocarcinoma, SIN3A is highly expressed in brain teratocarcinoma. So, it is likely that anti-mSin3A Monoclonal antibody M01203 will work for WB with brain teratocarcinoma.
Boster Scientific Support
Answered: 2019-12-24
Question
What is the sample target on Western blot analysis in your product image for M01203?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-MSin3A Rabbit Monoclonal Antibody (M01203) expression in (1) K562 cell lysate; (2) RAW 264.7 cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
I was wanting to use your anti-mSin3A Monoclonal antibody for WB for rat brain teratocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain teratocarcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
It shows on the product datasheet, M01203 anti-mSin3A Monoclonal antibody has been tested for IF, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain teratocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain teratocarcinoma using anti-mSin3A Monoclonal antibody M01203. Let me know if you need anything else.
Z. Jones
Verified customer
Asked: 2017-09-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-28
Question
My lab would like to test anti-mSin3A Monoclonal antibody M01203 on rat brain teratocarcinoma for research purposes, then I may be interested in using anti-mSin3A Monoclonal antibody M01203 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Jones
Verified customer
Asked: 2017-05-30
Answer
The products we sell, including anti-mSin3A Monoclonal antibody M01203, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-30
Question
We are currently using anti-mSin3A Monoclonal antibody M01203 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on horse tissues as well?
K. Taylor
Verified customer
Asked: 2015-10-26
Answer
The anti-mSin3A Monoclonal antibody (M01203) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-26
