Product Info Summary
SKU: | A00336 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-MTHFR Antibody
SKU/Catalog Number
A00336
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MTHFR Antibody catalog # A00336. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MTHFR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00336)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MTHFR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00336 is reactive to MTHFR in Human, Monkey, Mouse
Applications
A00336 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
74.597kDa
Background of MTHFR
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
Patricia Giraldo, Nucleic Acids Res., Nov 2003; 31: 6290 - 6305.
R Halaban, J. Cell Biol., Aug 1983; 97: 480.
Hee-Young Park, J. Biol. Chem., Jun 1999; 274: 16470.
BB Fuller, J. Biol. Chem., Mar 1987; 262: 4024 - 4033.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-MTHFR Antibody A00336
Western Blot (WB) analysis of specific cells using MTHFR polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MTHFR (Source: Uniprot.org, NCBI)
Gene Name
MTHFR
Full Name
Methylenetetrahydrofolate reductase
Weight
74.597kDa
Superfamily
methylenetetrahydrofolate reductase family
Alternative Names
EC 1.5.1.20; methylenetetrahydrofolate reductase (NAD(P)H)5,10-methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase MTHFR methylenetetrahydrofolate reductase methylenetetrahydrofolate reductase|5,10-methylenetetrahydrofolate reductase (NADPH)|methylenetetrahydrofolate reductase (NAD(P)H)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTHFR, check out the MTHFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTHFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MTHFR Antibody (A00336)
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No publications found for A00336
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