Product Info Summary
SKU: | M00336 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Mouse |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MTHFR Monoclonal Antibody
SKU/Catalog Number
M00336
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MTHFR Monoclonal Antibody catalog # M00336. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MTHFR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00336)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5D3
Isotype
IgG
Immunogen
Purified recombinant fragment of human MTHFR expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00336 is reactive to MTHFR in Human, Rat
Applications
M00336 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
74.597kDa
Background of MTHFR
Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. ELISA validation of MTHFR using Anti-MTHFR Monoclonal Antibody (M00336).
ELISA analysis of MTHFR antibody.Click image to see more details
Figure 2. Immunohistochemistry validation of MTHFR using Anti-MTHFR Monoclonal Antibody (M00336).
Immunohistochemistry (IHC) analysis of paraffin-embedded Lung Cancer Tissues with DAB staining using MTHFR Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-MTHFR Monoclonal Antibody M00336
Western Blot (WB) analysis using MTHFR Monoclonal Antibody against HEK293 (1) and MTHFR-hIgGFc transfected HEK293 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MTHFR (Source: Uniprot.org, NCBI)
Gene Name
MTHFR
Full Name
Methylenetetrahydrofolate reductase
Weight
74.597kDa
Superfamily
methylenetetrahydrofolate reductase family
Alternative Names
EC 1.5.1.20; methylenetetrahydrofolate reductase (NAD(P)H)5,10-methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase MTHFR methylenetetrahydrofolate reductase methylenetetrahydrofolate reductase|5,10-methylenetetrahydrofolate reductase (NADPH)|methylenetetrahydrofolate reductase (NAD(P)H)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTHFR, check out the MTHFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTHFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MTHFR Monoclonal Antibody (M00336)
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No publications found for M00336
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Recommended Resources
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Customer Reviews
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Customer Q&As
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