Anti-MUC5B Antibody Picoband™

Product Name

Anti-MUC5B Antibody Picoband™

View all MUC5B Antibodies

SKU/Catalog Number

A00719-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MUC5B Antibody Picoband™ catalog # A00719-2. Tested in ELISA, IHC applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MUC5B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00719-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MUC5B recombinant protein (Position: A5688-V5762).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00719-2 is reactive to MUC5B in Human

Applications

A00719-2 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

596.34kDa

Background of MUC5B

Mucin 5B (MUC5B/Muc5b) is a protein that in humans is encoded by the MUC5B gene and by Muc5b gene in mouse. This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. IHC analysis of MUC5B using anti-MUC5B antibody (A00719-2).
MUC5B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC5B Antibody (A00719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 2. IHC analysis of MUC5B using anti-MUC5B antibody (A00719-2).
MUC5B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC5B Antibody (A00719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For MUC5B (Source: Uniprot.org, NCBI)

Alternative Names

high molecular weight salivary mucin MG1; mucin 5, subtype B, tracheobronchial; mucin 5B, oligomeric mucus/gel-forming; sublingual gland mucin; tracheobronchial MUC5B MG1, MUC-5B, MUC5, MUC9 mucin 5B, oligomeric mucus/gel-forming mucin-5B|cervical mucin MUC5B|high molecular weight salivary mucin MG1|mucin 5, subtype B, tracheobronchial|sublingual gland mucin

For more info on MUC5B, check out the MUC5B Infographic

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In this infographic, you will see the following information for MUC5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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